Fluorescent aptasensor based on target-induced hairpin conformation switch coupled with nicking enzyme-assisted signal amplification for detection of beta-amyloid oligomers in cerebrospinal fluid.

Wan-Chen Chuang, Chun-Hsien Chen, Tsai-Hui Duh, Yen-Ling Chen

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Wan-Chen Chuang *Department of Chemistry and Biochemistry, National Chung Cheng University, 621301, Chia-Yi, Taiwan.

Chun-Hsien Chen *Department of Chemistry and Biochemistry, National Chung Cheng University, 621301, Chia-Yi, Taiwan.

Tsai-Hui DuhDepartment of Medicinal and Applied Chemistry, College of Life Science, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.

Yen-Ling ChenDepartment of Chemistry and Biochemistry, National Chung Cheng University, 621301, Chia-Yi, Taiwan. yelichen@ccu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A fluorescent aptasensor was developed based on target-induced hairpin conformation switch coupled with nicking enzyme-assisted signal amplification (NESA) to detect the oligomeric form of ß-amyolid peptide (AβO) in cerebrospinal fluid. The hairpin DNA probe (HP) was specifically designed to recognize AβO. When AβO is present in the sensing system, it induces an HP conformational switch and triggers the NESA reaction. After evaluating the parameters of the aptasensor system, we selected Hairpin10, which has a 10-nucleotide extended sequence, as the hairpin sequence that interacts with AβO. The quantitative linear range of the proposed aptasensor is from 11.3 to 113 ng mL

Indexed as

Amyloid beta-PeptidesAptamers, NucleotideBiosensing TechniquesFluorescent DyesDNA ProbesHumansInverted Repeat SequencesLimit of DetectionNucleic Acid ConformationSpectrometry, FluorescenceAmyloid beta-PeptidesAptamers, NucleotideDNA ProbesFluorescent DyesAlzheimer`s diseaseAβ oligomersFluorescent aptasensorNicking enzyme-assisted signal amplificationTarget-induced hairpin conformation switch

Identifiers

PMID39804483

DOI10.1007/s00604-024-06943-8

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.