Evidence map›Paper›PMID 39803431›Full record

ArticlebioRxiv : the preprint server for biology2024

Cryo-EM structure of the conjugation H-pilus reveals the cyclic nature of the TrhA pilin.

Naito Ishimoto, Joshua L C Wong, Nanki Singh, Sally Shirran, Shan He, Chloe Seddon, Olivia Wright-Paramio, Carlos Balsalobre, Ravi R Sonani, Abigail Clements and 3 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Naito IshimotoRutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Joshua L C WongDepartment of Life Sciences, Imperial College London, London, UK.
Nanki SinghRutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Sally ShirranBSRC Mass Spectrometry & Proteomics Facility, University of St Andrews, St Andrews, UK.
Shan HeDepartment of Life Sciences, Imperial College London, London, UK.
Chloe SeddonRutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Olivia Wright-ParamioDepartment of Life Sciences, Imperial College London, London, UK.
Carlos BalsalobreDepartment de Genètica, Microbiologia i Estadística, Facultat de Biologia. Universitat de Barcelona, Barcelona, Spain.
Ravi R SonaniDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.
Abigail ClementsRutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.ORCID 0000-0003-2285-777X
Edward H EgelmanDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.
Gad FrankelDepartment of Life Sciences, Imperial College London, London, UK.
Konstantinos BeisRutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.

Funding

Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic ResolutionR35GM122510 · NIGMS · UNIVERSITY OF VIRGINIA · PI EDWARD H. EGELMAN · 2017 to 2026
$7.3M
NIGMS NIH HHS R35 GM122510
6 · The paper itself

Abstract

Conjugation, the major driver of the spread of antimicrobial resistance genes, relies on a conjugation pilus for DNA transfer. Conjugative pili, such as the F-pilus, are dynamic tubular structures, composed of a polymerized pilin, that mediate the initial donor-recipient interactions, a process known as mating pair formation (MPF). IncH are low-copy-number plasmids, traditionally considered broad host range, which are found in bacteria infecting both humans and animals. The reference IncHI1 plasmid R27, isolated from

Indexed as

conjugationconjugation piluscyclizationmass spectrometrymating pair formation

Identifiers

PMID39803431
PMCPMC11722321

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.