Evidence map›Paper›PMID 39803320›Full record

ArticleBio-protocol2025

Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine.

Enrique Gamero-Estevez, Inga Viktoria Hensel, Michelle Steinhauer, Olivia Müllertz, Elizaveta Savochkina, Ibrahim Murathan Sektioglu, Bilgenaz Stoll, Shaghayegh Derakhshani, Sarah Devriese, Kyungbo Kim and 1 more

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Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Enrique Gamero-EstevezBioMed X Institute, Heidelberg, Germany.
Inga Viktoria HenselBioMed X Institute, Heidelberg, Germany.
Michelle SteinhauerBioMed X Institute, Heidelberg, Germany.
Olivia MüllertzBioMed X Institute, Heidelberg, Germany.
Elizaveta SavochkinaBioMed X Institute, Heidelberg, Germany.
Ibrahim Murathan SektiogluBioMed X Institute, Heidelberg, Germany.
Bilgenaz StollBioMed X Institute, Heidelberg, Germany.
Shaghayegh DerakhshaniBioMed X Institute, Heidelberg, Germany.
Sarah DevrieseBioMed X Institute, Heidelberg, Germany.
Kyungbo KimBioMed X Institute, Heidelberg, Germany.
Martin Resnik-DocampoBioMed X Institute, Heidelberg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The human intestine plays a pivotal role in nutrient absorption and immune system regulation. Along the longitudinal axis, cell-type composition changes to meet the varying functional requirements. Therefore, our protocol focuses on the processing of the whole human intestine to facilitate the analysis of region-specific characteristics such as tissue architecture and changes in cell populations. We describe how to generate a biobank that can be used to isolate specific immune cell subtypes, generate organoid lines, and establish autologous immune cell-organoid co-cultures. Key features • Dissection and tissue analysis of whole human intestines. • Cryopreservation for biobank generation. • Optimized protocols for the isolation of epithelial and immune cells. • Autologous co-culture of organoids and lamina propria-derived immune cells.

Indexed as

Autologous co-cultureBiobankCryopreservationImmune cell isolationIntestinal immune organoidsIntestinal organoidLamina propriaTissue processingWhole gut collection

Identifiers

PMID39803320
PMCPMC11717717

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.