ArticlePlastic and reconstructive surgery. Global open2025
Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential.
Article in Plastic and reconstructive surgery. Global open, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Effect of Cannula Fenestration Area on Fat Graft Architecture and Histology: A Double-Blind, Split-Body Clinical Trial.Aesthetic plastic surgery · 2026Trial
- From elective protocols to emergency reality: rethinking adipose tissue harvesting for stromal vascular fraction applications.Stem cells translational medicine · 2026Review
- Fat harvesting protocol for enhanced stem cell viability - pilot study.Clinics (Sao Paulo, Brazil) · 2026Article
- Strategic application of multilayer fat grafting in facial rejuvenation: a retrospective study.Frontiers in surgery · 2026Article
- Stemness and clinical performance of water-jet technology: A translational study in breast reconstruction.JPRAS open · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and centrifugation on the viability of stromal cells and ASCs in lipoaspirate. Methods: Fat was harvested from patients undergoing fat grafting using 2 types of liposuction cannula: (A) a 3-mm blunt tip cannula with 3 smooth holes and (B) a 2.4-mm, sharp point port, multihole blunt tip cannula. Fat from cannula B underwent different processing methods: no centrifugation, 300 Results: Lipoaspirates from 21 patients were analyzed. The mean stromal cell counts were 0.937 × 10 Conclusions: Fat harvesting using cannulas A and B showed no significant difference in stromal cell yield or viability. Handheld syringe liposuction preserved stromal vascular fraction cell and ASC viability. Centrifugation at different speeds did not significantly affect stromal cell viability.
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Registered trials
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