Evidence map›Paper›PMID 39801456›Full record

ArticleCancer medicine2025

Metagenomic Profiling of Oral Microbiome Dynamics During Chemoradiotherapy in Head and Neck Squamous Cell Carcinoma Patients.

Dominique A Torozan, Cédric Christian Laczny, Kirsten Roomp, Paul Wilmes, Jochen Fleckenstein, Jochen G Schneider

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Article in Cancer medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Dominique A TorozanDepartment of Radiotherapy and Radiation Oncology, University Hospital, Saarland University, Homburg, Germany.ORCID https://orcid.org/0009-0004-0546-2519
Cédric Christian LacznyLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.ORCID https://orcid.org/0000-0002-1100-1282
Kirsten RoompLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.ORCID https://orcid.org/0000-0002-4394-7494
Paul WilmesLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.ORCID https://orcid.org/0000-0002-6478-2924
Jochen FleckensteinDepartment of Radiotherapy and Radiation Oncology, University Hospital, Saarland University, Homburg, Germany.
Jochen G SchneiderLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.ORCID https://orcid.org/0000-0003-2139-0602

Funding

H2020 European Research Council 863664
6 · The paper itself

Abstract

backgroundWe explored the interaction between the oral microbiome and the development of radiation-induced mucositis in patients with head and neck squamous cell cancer (HNSCC) undergoing chemoradiotherapy (CRT). We prospectively studied the oral microbiome and compared it to healthy controls. Additionally, we compared patients with low-grade (LGM) vs. high-grade mucositis (HGM).

methodsTen HNSCC patients scheduled for CRT were included. Saliva samples were characterized prior to, during, and nine months after CRT using metagenomic sequencing. We similarly characterized samples from seven healthy controls. We assessed alpha and beta diversity and examined abundances at different taxonomic levels between (sub)groups.

resultsPatients exhibited significantly reduced alpha diversity compared to controls at all times (p ⟨ 0.05). Differential abundance of taxa between patients and controls was observed at baseline. In patients, the relative abundance of Staphylococcus aureus and Escherichia coli increased significantly during CRT. Capnocytophaga spp. was associated with the definitive CRT patients' subgroup. At baseline, two fungal families (Melampsoraceae and Herpotrichiellaceaea) were more abundant in patients who later developed HGM. No differentially abundant taxa were found between LGM vs. HGM during irradiation.

conclusionOur findings support the hypothesis that CRT, as well as HNSCC itself, influences the composition of the oral microbiome. Microbial markers found in patients who later developed HGM should be evaluated using independent cohorts to qualify their specific biomarker potential.

Indexed as

ChemoradiotherapyHead and Neck NeoplasmsMicrobiotaMouthSquamous Cell Carcinoma of Head and NeckAdultAgedBacteriaFemaleHumansMaleMetagenomeMetagenomicsMiddle AgedProspective StudiesSalivachemoradiotherapyhead and neck cancermetagenomic sequencingoral microbiomeradiation‐induced oral mucositis

Identifiers

PMID39801456
PMCPMC11725981

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