Evidence map›Paper›PMID 39801355›Full record

ReviewQuarterly reviews of biophysics2025

Structural biology inside multicellular specimens using electron cryotomography.

Ido Caspy, Zhexin Wang, Tanmay A M Bharat

Abstract readReview
In one paragraph

Review in Quarterly reviews of biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ido CaspyStructural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, UK.ORCID 0000-0003-1981-1017
Zhexin WangStructural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, UK.ORCID 0000-0002-4256-1143
Tanmay A M BharatStructural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, UK.ORCID 0000-0002-0168-0277

Funding

Engineering and Physical Sciences Research Council EP/V026623/1European Molecular Biology Organization ALTF 92-2022European Molecular Biology Organization YIPLeverhulme Trust Philip Leverhulme PrizeLister Institute of Preventive Medicine Lister PrizeMedical Research Council MC_UP_1201/31Wellcome Trust 225317Wellcome Trust 225317/Z/22/Z
6 · The paper itself

Abstract

The electron cryomicroscopy (cryo-EM) resolution revolution has shifted structural biology into a new era, enabling the routine structure determination of macromolecular complexes at an unprecedented rate. Building on this, electron cryotomography (cryo-ET) offers the potential to visualise the native three-dimensional organisation of biological specimens, from cells to tissues and even entire organisms. Despite this huge potential, the study of tissue-like multicellular specimens via cryo-ET still presents numerous challenges, wherein many steps in the workflow are being developed or in urgent need of improvement. In this review, we outline the latest techniques currently utilised for

Indexed as

Cryoelectron MicroscopyElectron Microscope TomographyAnimalsHumansImage Processing, Computer-Assistedcryo-EMcryo-ETFIB-SEMfocused ion beam millingin situ imagingstructural biologysubtomogram averagingtissue ultrastructurevitrificationvolume imaging

Identifiers

PMID39801355
PMCPMC7617309

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.