Evidence map›Paper›PMID 39801333›Full record

ArticleEuropean journal of public health2025

Molecular diagnosis and phylogenetic analysis of a Middle East respiratory syndrome coronavirus human case in Jordan.

Ehab A Abu-Basha, Zuhair B Ismail, Mohammad H Alboom, Ayesha Alkofahi, Basil H Amarneh, Omar Al-Omari, Alaa Fahmawi, Abdullah Alshammari, Mais Lakaideh, Shereen Shaban and 8 more

Abstract readCase Reports
In one paragraph

Article in European journal of public health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. MERS-COV in the Middle East, a one health concept approach.One health (Amsterdam, Netherlands) · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Ehab A Abu-BashaJordan University of Science and Technology, Irbid, Jordan.
Zuhair B IsmailJordan University of Science and Technology, Irbid, Jordan.
Mohammad H AlboomJordan University of Science and Technology, Irbid, Jordan.
Ayesha AlkofahiJordan University of Science and Technology, Irbid, Jordan.
Basil H AmarnehJordan University of Science and Technology, Irbid, Jordan.
Omar Al-OmariJordan University of Science and Technology, Irbid, Jordan.
Alaa FahmawiJordan University of Science and Technology, Irbid, Jordan.
Abdullah AlshammariJordan University of Science and Technology, Irbid, Jordan.
Mais LakaidehJordan University of Science and Technology, Irbid, Jordan.
Shereen ShabanJordan University of Science and Technology, Irbid, Jordan.
Bilal Al-OmariJordan University of Science and Technology, Irbid, Jordan.
Hani TalafhaJordan University of Science and Technology, Irbid, Jordan.
Zaidoun HijazeenJordan University of Science and Technology, Irbid, Jordan.
Yasmin DaradkehJordan University of Science and Technology, Irbid, Jordan.
Rabeh El-SheshenyDivision of Environmental Research, National Research Centre, Giza, Egypt.
Ghazi KayaliHuman Link, Dubai, United Arab Emirates.
Whitney BaggeEcoHealth Alliance, New York, NY, United States.
William B KareshEcoHealth Alliance, New York, NY, United States.

Funding

Department of Defense, Defense Threat Reduction AgencyJordan University of Science and Technology 32/2021
6 · The paper itself

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an important zoonotic pathogen. The aim of this paper is to report one polymerase chain reaction (PCR)-positive case of MERS-CoV in a 27-year-old man who was involved in a nationwide longitudinal surveillance study of certain zoonotic diseases in Jordan including MERS-CoV. Whole-blood and nasal swab samples were collected from the man and five camels in the vicinity of his living area. The samples were subjected to enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV-specific antibodies and MERS-CoV genetic material, respectively. Genomic sequencing and phylogenetic analysis were also performed to detect similarities with known strains of the virus in the region. In January 2021, an ongoing surveillance study detected a MERS-CoV-positive nasal swab sample from an asymptomatic male and camels using RT-PCR. Phylogenetically, the MERS-CoV isolated in this case belonged to clade B and is clustered with other strains originating in the Arabian Peninsula. The case report represents the first PCR-positive case of MERS-CoV in an asymptomatic individual in Jordan, indicating active circulation of the virus within the population.

Indexed as

Coronavirus InfectionsMiddle East Respiratory Syndrome CoronavirusAdultAnimalsCamelusHumansJordanMalePhylogeny

Identifiers

PMID39801333
PMCPMC11725956

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.