ArticleMolecules and cells2025
Optimization of FRET imaging in Arabidopsis protoplasts.
Article in Molecules and cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Molecules and Cells 2025, highlights from molecules to organisms.Molecules and cells · 2026Article
- Editorial: MiniResource papers from Molecules and Cells.Molecules and cells · 2026Article
- Calcium hydroxyapatite suppresses extracellular matrix-related gene expression by modulating Piezo1-associated CaAnimal cells and systems · 2026Article
- Understanding Crosstalk Between Phosphate and Immune-Related Signaling in Rice andPlants (Basel, Switzerland) · 2025Article
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Authors and funding
4 authors.
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Abstract
Recent advancements in fluorescence-based biosensor technologies have enabled more precise and accurate Förster resonance energy transfer (FRET) imaging within Agrobacterium-mediated plant transformation systems. However, the application of FRET imaging in plant tissues remains hindered by significant challenges, particularly the time-intensive process of generating transgenic lines and the complications arising from tissue autofluorescence. In contrast, protoplast-based FRET imaging offers a rapid and efficient platform for functional screening and analysis, making it an essential tool for plant research. Nevertheless, conventional protoplast-based FRET approaches are often limited by background interference, inconsistent imaging conditions, and difficulties in quantitative analysis. Here, we present a systematic optimization of imaging conditions using the calcium biosensor D3cpv, addressing these limitations to improve both precision and efficiency in protoplast-based FRET imaging. This work serves as a practical guide for streamlining FRET imaging workflows and maximizing the utility of biosensors in plant cell studies.
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