ArticleMikrochimica acta2025
A PDMS/chitosan/MPMs composite film based on multi-field coupling enhancement for African swine fever virus P72 protein detection.
Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
African swine fever (ASF) is an acute hemorrhagic disease in pigs caused by the African swine fever virus (ASFV), which has a high mortality rate and brought great damage to global pig farming industry. At present, there is no effective treatment or vaccine to combat ASFV infection, so early detection of ASFV has become particularly important. Therefore, the PDMS/chitosan/MPMs composite film was proposed to detect ASFV P72. PDMS mixed with chitosan particles were used to provide biological modification sites for the ASFV P72 antibody, which simplified the functional modification process. The specific binding of antigen and antibody leads to the mechanical deformation of the composite film, which was observed through optical method to change the microsphere spacing. The polystyrene microsphere (PMs) spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the PDMS/chitosan/PMs with a simple laser pointer. The conductive layer formed by AgNWs realize the transformation of biological signals to electrical signals. By conjugating PMs with Fe
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