Evidence map›Paper›PMID 39798570›Full record

ArticleMolecular cell2025

RBBP6 anchors pre-mRNA 3' end processing to nuclear speckles for efficient gene expression.

Yoseop Yoon, Elodie Bournique, Lindsey V Soles, Hong Yin, Hsu-Feng Chu, Christopher Yin, Yinyin Zhuang, Xiangyang Liu, Liang Liu, Joshua Jeong and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. A unified photosensitizer platform forbioRxiv : the preprint server for biology · 2026
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Nuclear speckles are regulatory hubs for viral and host mRNA expression during HSV-1 infection.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Yoseop YoonDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Elodie BourniqueDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Lindsey V SolesDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Hong YinDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Hsu-Feng ChuDepartment of Biological Sciences, Columbia University, New York, NY 10027, USA.
Christopher YinDepartment of Electrical and Computer Engineering, University of Washington, Seattle, Seattle, WA 98195, USA.
Yinyin ZhuangDepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA.
Xiangyang LiuDepartment of Biological Sciences, Columbia University, New York, NY 10027, USA.
Liang LiuDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Joshua JeongDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Clinton YuDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA.
Marielle ValdezDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Lusong TianDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Lan HuangDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA.
Xiaoyu ShiDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697, USA; Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA.
Georg SeeligDepartment of Electrical and Computer Engineering, University of Washington, Seattle, Seattle, WA 98195, USA; Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, Seattle, WA 98195, USA.
Fangyuan DingDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Liang TongDepartment of Biological Sciences, Columbia University, New York, NY 10027, USA.
Rémi BuissonDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Yongsheng ShiDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA. Electronic address: yongshes@uci.edu.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Structural and functional studies of mRNA processing, stability and quality controlR35GM118093 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI LIANG TONG · 2016 to 2026
$8.5M
mRNA alternative polyadenylation in B cell developmentR01AI170840 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Roger Sciammas, Yongsheng Shi · 2022 to 2026
$4.1M
Supplement: Advancing Proteomics Technologies to Decipher the Ubiquitin-Proteasome SystemR35GM145249 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Lan Huang · 2022 to 2026
$3.2M
Gel-based Optical-isolation Single-Cell 3D Spatial MultiomicsDP2GM150017 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI SHI, XIAOYU · 2022 to 2025
$2.6M
Molecular Mechanisms of APOBEC-Induced Mutagenesis in CancerR37CA252081 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Remi Buisson · 2021 to 2026
$2.5M
Mechanisms and regulation of mRNA 3' processingR35GM149294 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Yongsheng Shi · 2023 to 2026
$2.1M
Zeiss LSM 980 NLO Confocal Microscope with Airyscan2 for UCI Optical Biology CoreS10OD032327 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI WARRIOR, RAHUL · 2022 to 2022
$600k
NF-KB regulation by the DNA damage responseR21ES036190 · NIEHS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BUISSON, REMI · 2025 to 2025
$432k
Role of APOBEC3B in the Innate Immune ResponseR21AI185033 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Remi Buisson · 2025 to 2026
$425k
NCI NIH HHS P30 CA062203NCI NIH HHS R37 CA252081NIAID NIH HHS R01 AI170840NIAID NIH HHS R21 AI185033NIEHS NIH HHS R21 ES036190NIGMS NIH HHS DP2 GM150017NIGMS NIH HHS R35 GM118093NIGMS NIH HHS R35 GM145249NIGMS NIH HHS R35 GM149294NIH HHS S10 OD032327
6 · The paper itself

Abstract

Pre-mRNA 3' processing is an integral step in mRNA biogenesis. However, where this process occurs in the nucleus remains unknown. Here, we demonstrate that nuclear speckles (NSs), membraneless organelles enriched with splicing factors, are major sites for pre-mRNA 3' processing in human cells. We show that the essential pre-mRNA 3' processing factor retinoblastoma-binding protein 6 (RBBP6) associates strongly with NSs via its C-terminal intrinsically disordered region (IDR). Importantly, although the conserved N-terminal domain (NTD) of RBBP6 is sufficient for pre-mRNA 3' processing in vitro, its IDR-mediated association with NSs is required for efficient pre-mRNA 3' processing in cells. Through proximity labeling analyses, we provide evidence that pre-mRNA 3' processing for over 50% of genes occurs near NSs. We propose that NSs serve as hubs for RNA polymerase II transcription, pre-mRNA splicing, and 3' processing, thereby enhancing the efficiency and coordination of different gene expression steps.

Indexed as

Cell NucleusRNA 3' End ProcessingRNA, MessengerRNA PrecursorsGene Expression RegulationHEK293 CellsHeLa CellsHumansIntrinsically Disordered ProteinsProtein BindingRNA-Binding ProteinsRNA Polymerase IIRNA Processing, Post-TranscriptionalRNA SplicingIntrinsically Disordered ProteinsRNA-Binding ProteinsRNA, MessengerRNA Polymerase IIRNA Precursorsbiomolecular condensatecleavage and polyadenylationgene expressionintrinsically disordered regionmembraneless organellenuclear specklephase separationpre-mRNA 3′ end processingtranscription termination

Identifiers

PMID39798570
PMCPMC11805622

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.