ArticleSTAR protocols2025
Protocol to identify endogenous proximal proteins using biotinylation by antibody recognition.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Improving recombinant antibody production using FcBAR: An in situ approach to detect and amplify protein-protein interactions.Metabolic engineering · 2025Article
- Improving Recombinant Antibody Production Using FcBAR: An In Situ Approach to Detect and Amplify Protein-Protein Interactions.bioRxiv : the preprint server for biology · 2025Article
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Authors and funding
4 authors.
Funding
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Abstract
Biotinylation by antibody recognition (BAR) is an antibody-based approach for mapping proximal protein interactions in cells. Here, we present a protocol to biotinylate and identify proximal proteins using BAR. We describe steps for defining proximity labeling reaction conditions, assessing enrichment using western blot, and sample preparation for mass spectroscopy analysis. We then detail procedures for data analysis and identifying proximal proteins. This approach differs from standard proximity labeling techniques, which rely on genetically engineered enzymes fused to the target protein. For complete details on the use and execution of this protocol, please refer to Rega et al.
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