Development of Automatic Method for Glucose Detection Based on Platinum Octaethylporphyrin Sol-Gel Film with Long-Term Stability.

In this study, an approach has been proposed in response to the urgent need for a sensitive and stable method for glucose detection at low concentrations. Platinum octaethylporphyrin (PtOEP) was chosen as the probe and embedded into the matrix material to yield a glucose-sensing film, i.e., Pt/TE-MTS, through a sol-gel process. The optical parameter (OP) was defined as the ratio of phosphorescence in the absence and presence of glucose, and the relationship between OP and glucose concentration (GC) was established in a theoretical way based on the Stern-Volmer equation and further obtained by photoluminescence measurement. OP exhibited a linear relationship with GC in a range of 0-720 μM. The time required by the photoluminescence of the film to reach equilibrium was measured to ensure the completion of the reaction, and it was found that the equilibrium time decreased as the GC increased. The photobleaching behavior and stabilization of the film were monitored, and the result showed that the film exhibited excellent resistance to photobleaching and was quite stable in an aqueous solution. Additionally, a LabVIEW-based GC-detection system was developed to achieve the practical application of the sensing film. In summary, the Pt/TE-MTS film exhibited high sensitivity in detecting the GC with excellent reproducibility, which is of high value in applications.