ArticleJournal of orthopaedic surgery and research2025
Validation and the role of PDK4 relevant to ferroptosis in degenerative lumbar disc disease.
Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Efficacy of radiofrequency ablation in the treatment of discogenic low back pain: a meta-analysis.Journal of orthopaedic surgery and research · 2025Pooled it
- Ferroptosis in musculoskeletal disorders: Emerging mechanisms and therapeutic opportunities (Review).International journal of molecular medicine · 2026Review
- FBXW7 induces apoptosis of nucleus pulposus cells to mediate intervertebral disc degeneration by regulating JNK expression.Journal of orthopaedic surgery and research · 2026Article
- Unilateral biportal endoscopy versus percutaneous endoscopic lumbar discectomy for far lateral disc herniation: a comparative study of clinical efficacy and learning curves.BMC musculoskeletal disorders · 2026Article
- Metabolic stress exacerbates Parkinson's disease pathology through mitochondrial dysfunction and ferroptosis.NPJ Parkinson's disease · 2026Article
- Clinical effects and prognostic factors of combined laminoplasty with keyhole decompression for multilevel cervical spondylotic myelopathy: analysis based on cervical sagittal parameters.BMC musculoskeletal disorders · 2026Article
- Multi-omics Mendelian randomization prioritizes BMP6 as a candidate therapeutic target in intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2026Article
- miR-145-5p combined with miR-140-5p regulates the differentiation of bone marrow mesenchymal stem cells into nucleus pulposus cells and its mechanism in the treatment of intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2026Article
- Associations between the serum follicle-stimulating hormone (FSH) levels and intervertebral disc degeneration severity during the menopausal transition.Journal of orthopaedic surgery and research · 2026Article
- Article
- Periostin as a diagnostic marker and therapeutic target for intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2025Article
- Exosomal miR-223-3p from mesenchymal stem cells targets FBXW7 to inhibit intervertebral disc degeneration: mechanism insights.Journal of orthopaedic surgery and research · 2025Article
- miR-302a-3p mitigates intervertebral disc degeneration progression through regulating EPHA2.Journal of orthopaedic surgery and research · 2025Article
- Targeting ICAM1 through LIF-mediated NF-κB/JAK2-STAT3 crosstalk protects nucleus pulposus from IL-1β-driven pyroptosis to ameliorate intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2025Article
- Transcriptomic insights into the anti-apoptotic effect of Tuina in rabbit model of intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2025Article
- Value of contrast-enhanced MRI for predicting resorption of ruptured lumbar disc herniation: a prospective study of 82 cases.Journal of orthopaedic surgery and research · 2025Article
- LncRNA MIR100HG induces degenerative changes in intervertebral disc degeneration nucleus pulposus cells by targeting miR-31-5p.Journal of orthopaedic surgery and research · 2025Article
- Obesity-induced NLRP3 inflammasome activation in nucleus pulposus cells accelerates intervertebral disk degeneration.Journal of orthopaedic surgery and research · 2025Article
- A novel classification and clinical evaluation of various morphologies of calcified lumbar disc herniation treated using unilateral biportal endoscopic technique.Journal of orthopaedic surgery and research · 2025Article
- Tie2-mediated CBL ubiquitination of EGFR underlies ultrasound-responsive silk fibroin/graphene oxide hydrogel-troxerutin therapy for intervertebral disc degeneration.Materials today. Bio · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFerroptosis was involved in the pathogenesis of intervertebral disc degeneration (IVDD). However, the exact mechanism of IVDD associated with ferroptosis still required deeper studies.
methodThe differentially expressed genes (DEGs) in rat lumbar disc tissue between the control and IVDD group treated with IL-1β were detected by RNA sequencing (RNA-seq). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on DEGs. We further screened the differential expressed ferroptosis-related genes (DEFRGs). Besides, a protein-protein interaction (PPI) network of DEFRGs was constructed by STRING database. The Cytoscape database identified significant modules and the hub genes. The loss function of PDK4 by siRNA inference was investigated in NPCs by CCK8 assay, ELISA assay, and the analysis of ferroptosis indicators.
resultDEGs were identified using RNA-seq. KEGG pathway analysis showed that these genes were mainly involved in Parkinson's disease, oxytocin signaling pathway, calcium ion signaling pathway, AMPK signaling pathway, and glucagon signaling pathway. Eight hub genes (including LDHA, PKM, EP300, EGFR, EGLN1, SCD, PDK4, and FABP4) were found by the PPI network and Cytoscape on a total of 25 ferroptosis-related genes that were identified in rat lumbar disc tissue after IVDD treatment. PDK4 silencing promoted NPCS proliferation, decreased the levels of the proinflammatory factors, and suppressed ferroptosis.
conclusionThe study suggested the potential roles of ferroptosis-related genes in IVDD and further revealed the role of PDK4 in the progression of IVDD.
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