ArticleBMC biotechnology2025
Metabolic studies of Ogataea polymorpha using nine different corn steep liquors.
Article in BMC biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Microbial synthesis and degradation of bio-based biodegradable plastics.Nature reviews. Microbiology · 2026Review
- Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms.Biotechnology for biofuels and bioproducts · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the fermentation industry, the demand to replace expensive complex media components is increasing for alternative nutrient sources derived from waste or side streams, such as corn steep liquor (CSL). However, the use of CSL is associated with common problems of side products, such as batch-to-batch variations and compositional inconsistencies. In this study, to detect batch-to-batch variations in CSL for Ogataea polymorpha cultivations, a "fingerprinting" system was developed by employing the Respiration Activity Monitoring System designed for shake flasks (RAMOS) and 96-well microtiter plates (µTOM).
resultsAt 2.5 g d.s./L CSL and 5 g/L glucose, a limitation by a secondary substrate, other than the carbon source, was observed. For this specific CSL medium, this limitation was caused by ammonium nitrogen and could be removed through targeted supplementation of ammonium sulphate. Under ammonium nitrogen limitation, O. polymorpha showed a change in morphology and developed a different cell size distribution. Increasing CSL storage times impaired O. polymorpha cultivation results. It was speculated that this observation is caused by micronutrient precipitation as sulfide salts. Through targeted nutrient supplementation, these limiting microelements were identified to be copper, iron and zinc.
conclusionsThis study shows the versatility of CSL as an alternative nutrient source for O. polymorpha cultivations. "Fingerprinting" of CSL batches allows for early screening. Fermentation inconsistencies can be eliminated by selecting the better performing CSL batches or by supplementing and improving an inferior CSL prior to large-scale productions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.