Evidence map›Paper›PMID 39792957›Full record

ArticlePLoS neglected tropical diseases2025

Insect-specific RNA viruses detection in Field-Caught Aedes aegypti mosquitoes from Argentina using NGS technology.

Lucas Ripoll, Javier Iserte, Carolina Susana Cerrudo, Damian Presti, José Humberto Serrat, Ramiro Poma, Federico Alejandro Javier Mangione, Gabriela Analía Micheloud, Verónica Viviana Gioria, Clara Inés Berrón and 3 more

Abstract read
In one paragraph

Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Vector competence acrossJournal of virology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lucas RipollLaboratorio de Ingeniería Genética y Biología Celular y Molecular-Área de virus de insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Quilmes, Buenos Aires, Argentina.ORCID https://orcid.org/0009-0003-4160-6850
Javier IserteLaboratorio de Bioinformática Estructural, Fundación Instituto Leloir, Ciudad de Buenos Aires, Buenos Aires, Argentina.
Carolina Susana CerrudoLaboratorio de Ingeniería Genética y Biología Celular y Molecular-Área de virus de insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Quilmes, Buenos Aires, Argentina.
Damian PrestiLaboratorio de Ingeniería Genética y Biología Celular y Molecular-Área de virus de insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Quilmes, Buenos Aires, Argentina.
José Humberto SerratPrograma de Zoonosis, Dirección General de Coordinación Epidemiológica-Ministerio de Salud Pública de Salta, Salta, Salta, Argentina.
Ramiro PomaUnidad de Conocimiento Traslacional Hospitalaria, Hospital Público Materno Infantil de Salta (UCT-HPMI)-CONICET, Salta, Salta, Argentina.
Federico Alejandro Javier MangionePrograma de Zoonosis, Dirección General de Coordinación Epidemiológica-Ministerio de Salud Pública de Salta, Salta, Salta, Argentina.
Gabriela Analía MicheloudLaboratorio de Virología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Santa Fe, Argentina.
Verónica Viviana GioriaLaboratorio de Virología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Santa Fe, Argentina.
Clara Inés BerrónLaboratorio de Virología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Santa Fe, Argentina.
M Paola ZagoUnidad de Conocimiento Traslacional Hospitalaria, Hospital Público Materno Infantil de Salta (UCT-HPMI)-CONICET, Salta, Salta, Argentina.
Cristina BorioLaboratorio de Ingeniería Genética y Biología Celular y Molecular-Área de virus de insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Quilmes, Buenos Aires, Argentina.
Marcos BilenLaboratorio de Ingeniería Genética y Biología Celular y Molecular-Área de virus de insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Quilmes, Buenos Aires, Argentina.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mosquitoes are the primary vectors of arthropod-borne pathogens. Aedes aegypti is one of the most widespread mosquito species worldwide, responsible for transmitting diseases such as Dengue, Zika, and Chikungunya, among other medically significant viruses. Characterizing the array of viruses circulating in mosquitoes, particularly in Aedes aegypti, is a crucial tool for detecting and developing novel strategies to prevent arbovirus outbreaks. In this study, we address the implementation of a sequencing and analysis pipeline based on the Oxford Nanopore Technologies MinION Mk1b system, for arboviral detection in field-caught mosquitoes from Argentina. Full genome of Humaita Tubiacanga Virus (HTV), Phasi Charoen-like Phasivirus (PCLV), Aedes aegypti totivirus (AaeTV) has been sequenced in three distinct regions of Argentina comprising Buenos Aires province, Santa Fe province and the northern province of Salta. Viral sequences enriched by SISPA and coupled with Nanopore sequencing can be a useful tool for viral surveillance, not only for detecting viruses that have a high impact on human and animal health, but also for detecting insect-specific viruses that could promote the transmission of arboviruses.

Indexed as

AedesMosquito VectorsRNA VirusesAnimalsArbovirusesArgentinaGenome, ViralHigh-Throughput Nucleotide SequencingPhylogeny

Identifiers

PMID39792957
PMCPMC11756794

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.