ArticleApplied biochemistry and biotechnology2025
Epoxy-Affixed ZIF-8/CS/Cellulase: a Sustainable Approach for Hydrolysis of Agricultural Waste to Reducing Sugars.
Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cellulase was effectively immobilized onto an epoxy-bound chitosan-modified zinc metal-organic framework (epoxy/ZIF-8/CS/cellulase) support, yielding a conjugation rate of 0.64 ± 0.02 mg/cm2 and retaining 80.01 ± 0.01% of its specific activity. The bare and cellulase-bound supports was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, atomic force microscopy and energy-dispersive X-ray spectroscopy. The immobilized enzyme exhibited optimal activity at pH 5.5 and a temperature of 70 ℃. The efficiency, stability and reactivity of the enzyme improved after immobilization, as evidenced by a decrease in activation energy, enthalpy and Gibbs free energy along with an increase in entropy change. The epoxy-affixed ZIF-8/CS/cellulase strip was successfully employed for rice husk hydrolysis achieving an impressive conversion efficiency of 95%. The method demonstrated a linear range from 0.1 to 0.9% (0.1 × 10
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