ArticleCommunications biology2025
Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The trial behind it
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Who cites it
5 citing papers in PubMed.
- Resurrection of chromosomes from frozen animals by single chromosome transfer into mouse oocytes.Scientific reports · 2026Article
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- The Consequences of DNA Damage in the Early Embryo Are Important for Practical Procedures in Assisted Reproduction.International journal of molecular sciences · 2025Review
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Abnormal chromosome segregation (ACS) in preimplantation embryos causes miscarriages. For a normal pregnancy, it is necessary to reduce ACS occurrences in embryos. However, the causes of such abnormalities are unclear because no method to extract the segregated chromosomes from the blastomeres for detailed analysis. This study attempted to extract micronuclei derived from segregated chromosomes of mouse embryos. Some micronuclei in blastomeres were bound to the nucleus by DNA cross-links, some were bound to tubulin, and about half of the micronuclei had major satellite regions. By depolymerizing the cytoskeleton of blastomeres with cytochalasin B and colcemid, some micronuclei could be extracted from blastomeres of ACS embryos using a glass needle of a micromanipulator. DNA-sequencing results of each extracted micronucleus revealed that chromosomes in micronuclei were randomly selected, usually only one, and often contained a portion rather than the full length of the chromosome. This study allows a detailed analysis of micronuclei and facilitates the mechanism of the causes of ACS in embryos.
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Registered trials
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