Article in American journal of physiology. Lung cellular and molecular physiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
7 authors.
Olivia S HarlowDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States.ORCID 0000-0001-8142-8698
Vijay Raaj RaviDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States.
Fang KeDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States.
Nathan L SandersPulmonary Center, Boston University Chobanian and Avedisian School of Medicine, Boston, Massachusetts, United States.
Elise ArmstrongPulmonary Center, Boston University Chobanian and Avedisian School of Medicine, Boston, Massachusetts, United States.
Joseph P MizgerdPulmonary Center, Boston University Chobanian and Avedisian School of Medicine, Boston, Massachusetts, United States.ORCID 0000-0002-4042-3169
Anukul T ShenoyDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States.ORCID 0000-0001-8861-2295
Funding
BIOLOGY OF THE LUNG--MULTIDISCIPLINARY PROGRAMT32HL007035 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton, JOSEPH P MIZGERD · 1985 to 2026
$24.3M
RESEARCH TRAINING IN IMMUNOLOGYT32AI007309 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI GUMMULURU, SURYARAM · 1988 to 2024
$7.4M
Molecular Mechanisms of Microbial Pathogenesis Training ProgramT32AI007528 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CARRUTHERS, VERNON BRUCE · 1998 to 2024
$6.8M
Pneumonia BiologyR35HL135756 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI MIZGERD, JOSEPH P · 2017 to 2023
$6.6M
Lung-resident antibacterial heterotypic immunityR01AI115053 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI MIZGERD, JOSEPH P · 2015 to 2023
$4.0M
Lung epithelial cell reprogramming by CD4 T cellsR00HL157555 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHENOY, ANUKUL T · 2023 to 2025
$747k
HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI) HL157555HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) T32AI007309NHLBI NIH HHS R00 HL157555NHLBI NIH HHS R35 HL135756NHLBI NIH HHS T32 HL007035NIAID NIH HHS R01 AI115053NIAID NIH HHS T32 AI007309NIAID NIH HHS T32 AI007528U-M | Horace H. Rackham School of Graduate Studies, University of Michigan (Rackham U-M)University of Michigan Medical School
6 · The paper itself
Abstract
Rigor and reproducibility are vital to scientific advancement. It is unclear whether a protocol optimized for tissue dissociation in one institution performs well universally. Here, we share our brand-new lab's experience with interinstitutional variability that led to the discovery that a protocol optimized for murine lung dissociation at Boston University (BU) fails to reproduce similar CD4
Indexed as
LungLymphocytesAnimalsB-LymphocytesCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCollagenasesMiceMice, Inbred C57BLReproducibility of ResultsCollagenasescollagenaseflow cytometrylung dissociationlymphocytesrigor and reproducibility
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
The mysterious case of missing lymphocytes: a cautionary tale of interinstitutional variability in outcomes of lung dissociation protocols. · full record | OpenQuestion