Evidence map›Paper›PMID 39786545›Full record

ArticleArchives of microbiology2025

Hybrid Pennisetum colonization by Bacillus megaterium BM18-2 labeled with green fluorescent protein (GFP) under Cd stress.

Nehal Kamal, Chen Qian, Huanhuan Hao, Juanzi Wu, Zhiwei Liu, Xiaoxian Zhong, Osama M Ghanem, Ali Salem, Zoltan Orban, Abdallah Elshawadfy Elwakeel and 2 more

Abstract read
In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Nehal Kamal *National Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China. nero_micro@yahoo.com.
Chen Qian *National Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China.
Huanhuan HaoCollege of Agro-grassland Science, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.
Juanzi WuNational Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China.
Zhiwei LiuNational Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China.
Xiaoxian ZhongNational Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China. xiaoxian@jaas.ac.cn.
Osama M GhanemSoil and Water Department, Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt.
Ali SalemCivil Engineering Department, Faculty of Engineering, Minia University, Minya, Egypt. salem.ali@mik.pte.hu.
Zoltan OrbanStructural Diagnostics and Analysis Research Group, Faculty of Engineering and Information Technology, University of Pécs, Pécs, Hungary.
Abdallah Elshawadfy ElwakeelAgricultural Engineering Department, Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt.
Samy F MahmoudDepartment of Biotechnology, College of Science, Taif University, Taif city, Saudi Arabia.
Alaa F SaidAgricultural Botany Department, Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Researchers have reported that Bacillus megaterium BM18-2 reduces Cd toxicity in Hybrid Pennisetum, but understanding the interaction between plants and associated endophytes is crucial for understanding phytoremediation strategies under heavy metal stress. The current study aims to monitor the colonization patterns of GFP-labeled endophytic bacteria BM18-2 on Hybrid Pennisetum grass. Additionally, it will monitor Cd's effect on plant bacterial colonization. Confocal laser scanning microscopy of plant roots infected with gfp tagged BM18-2 revealed that the bacterium colonised root hairs and epidermal cells at the early stage of colonization, and over time, the bacteria penetrated to the internal tissues following their colonization of the stem and leaf. The roots, stems, and leaves of H. Pennisetum grown in Cd-contaminated soil contained a higher number of bacteria than those grown in normal soil. The result of Cd translocation indicated the condensation of heavy metals in the root cells and stem, while no Cd was found in the leaf. The study will also look for the enzymatic activity of bacteria BM18-2 and use Leadmium Green AM dye to track how Cd is taken up and moved through the plant. The enzymatic activity results showed that BM18-2 can produce catalase and amylase, but did not record any cellulase or lipase activity. As a result, the pattern of useful endophytic BM18-2 colonization through H. Pennisetum grass will aid in the application and maintenance of these bacteria in farming, and it presents new opportunities for the development of innovative strategies in the fields of agriculture and biotechnology.

Indexed as

Bacillus megateriumBiodegradation, EnvironmentalCadmiumGreen Fluorescent ProteinsPennisetumPlant RootsSoil PollutantsEndophytesMetals, HeavyPlant LeavesSoil MicrobiologyCadmiumGreen Fluorescent ProteinsMetals, HeavySoil PollutantsBacillus Megaterium BM18-2CadmiumGFPHybrid Pennisetum

Identifiers

PMID39786545
PMCPMC11717813

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.