ArticleTransgenic research2025
Expression of Agrobacterium Isopentenyl transferase (IPT) gene in wheat improves drought tolerance.
Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Biotechnological modification of the cytokinin regulatory system to improve drought and heat tolerance in the major crops.Plant cell reports · 2026Review
- Deciphering the cucumber ATG6 and its promoter to enhance abiotic stress tolerance and drive stress-responsive transgene expression in plants.Transgenic research · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
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Authors and funding
3 authors.
Funding
Abstract
Drought, as an abiotic stressor, globally limits cereal productivity, leading to early aging of leaves and lower yields. The expression of the isopentenyl transferase (IPT) gene, which is involved in cytokinin (CK) biosynthesis, can delay drought-induced leaf senescence. In this study, the Agrobacterium Isopentenyl transferase (IPT) gene was introduced into two local hexaploid wheat cultivars, NR-421 and FSD-2008. The expression cassette was developed containing the IPT gene under transcriptional regulation of the stress-inducible promoter 'Dehydrin,' sourced from Hordeum vulgare. The gene expression cassette was assembled in pSB219M, a modified transformation vector for monocots, equipped with both an antibiotic (spectinomycin) and an herbicide selection marker (BASTA). Initial screening of transgenic plants involved BASTA selection (2 and 3 mg/L) and was subsequently confirmed through PCR analysis. The transformation efficiencies of NR-421 and FSD-2008 were 0.4% and 0.3%, respectively. The qRT-PCR analysis under stress conditions showed a 13.5-fold higher expression of the IPT gene in T
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