Evidence map›Paper›PMID 39783851›Full record

ArticleJournal of extracellular vesicles2025

A simple polydopamine-based platform for engineering extracellular vesicles with brain-targeting peptide and imaging probes to improve stroke outcome.

Xiaojing Shi, Lu Zhang, Shengju Wu, Chunfu Zhang, Muyassar Mamtilahun, Yongfang Li, Zhijun Zhang, Changjing Zuo, Fengzhen Cui, Wanlu Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaojing ShiShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Lu ZhangDepartment of Nuclear Medicine, Changhai Hospital Affiliated to Naval Medical University, Shanghai, China.
Shengju WuShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Chunfu ZhangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Muyassar MamtilahunShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Yongfang LiDepartment of Rehabilitation Medicine, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, China.
Zhijun ZhangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Changjing ZuoDepartment of Nuclear Medicine, Changhai Hospital Affiliated to Naval Medical University, Shanghai, China.
Fengzhen CuiShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Wanlu LiShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Guo-Yuan YangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Yaohui TangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.ORCID https://orcid.org/0000-0002-9603-2650

Funding

Fundamental Research Funds for the Central Universities YG2023ZD02National Key Research and Development Program of China 2022YFA1603600National Natural Science Foundation of China 81901804National Natural Science Foundation of China 82172529National Natural Science Foundation of China 82271320National Natural Science Foundation of China 82371307National Natural Science Foundation of China 82472590Shanghai Municipal Education Commission ZXWH1082101Shanghai Sailing Program 23YF1420700
6 · The paper itself

Abstract

Extracellular vesicles (EVs) have shown great potential for treating various diseases. Translating EVs-based therapy from bench to bedside remains challenging due to inefficient delivery of EVs to the injured area and lack of techniques to visualize the entire targeting process. Here we developed a dopamine surface functionalization platform that facilitates easy and simultaneous conjugation of targeting peptide and multi-mode imaging probes to the surface of EVs. Utilizing this platform we concurrently modified M2 microglia-derived EVs (M2-EVs) with neuronal targeting peptide rabies virus glycoprotein peptide 29 (RVG29) and multi-modal imaging tracers, resulting in the targeted delivery of M2-EVs to stroke mice brain and enabled the dynamic visualization of the targeting process from whole-body to cellular levels. We determined that intra-arterial injection achieved the highest efficiency of targeted delivery of engineered EVs to the stroke mice brain, improved therapeutic efficacy by reducing neuronal apoptosis. Mechanistically, EVs miRNA array revealed that a number of anti-apoptosis related miRNAs were significantly up-regulated, including miR-221-3p and miR-423-3p, both exerted anti-apoptotic effects through p38/ERK signalling pathways in stroke. Overall, this platform provides a facile and powerful tool for multifunctional engineering of EVs for multiscale therapeutic evaluation and enhancement of EV-based therapy, with valuable prospects for clinical translation.

Indexed as

BrainExtracellular VesiclesIndolesMicroRNAsPolymersStrokeAnimalsApoptosisDisease Models, AnimalGlycoproteinsHumansMaleMiceMice, Inbred C57BLMicrogliaPeptide FragmentsGlycoproteinsIndolesMicroRNAsPeptide FragmentsPeptidespolydopaminePolymersrabies virus glycoprotein peptideViral Proteinsextracellular vesicleIschemic strokeMultifunctional engineeringTargeted delivery

Identifiers

PMID39783851
PMCPMC11714163

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.