Evidence map›Paper›PMID 39780724›Full record

ArticleThe journal of physical chemistry. B2025

The Expression, Purification, Spectroscopic Characterization, and Membrane Topology Classification of KCNE4 from Recombinant

Alison Bates, Ilsa Miller, Elizabeth M Travis, Indra D Sahu, Andrew Morris, Robert M McCarrick, Carole Dabney-Smith, Gary A Lorigan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physical chemistry. B, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alison BatesDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.ORCID 0009-0005-7313-0345
Ilsa MillerDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Elizabeth M TravisDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Indra D SahuDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.ORCID 0000-0002-7333-0356
Andrew MorrisDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Robert M McCarrickDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Carole Dabney-SmithDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Gary A LoriganDepartment of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.ORCID 0000-0002-2395-3459

Funding

EPR Spectroscopic Studies of Membrane Proteins-Diversity SupplementR35GM126935 · NIGMS · MIAMI UNIVERSITY OXFORD · PI GARY A LORIGAN · 2018 to 2026
$3.3M
NIGMS NIH HHS R35 GM126935
6 · The paper itself

Abstract

Members of the KCNE family are accessory subunits that modulate voltage-gated potassium channels. One member, KCNE4, has been shown to inhibit the potassium ion current in these channels. However, little is known about the structure, dynamics, and mode of inhibition of KCNE4, likely due to challenges in overexpressing and purifying the protein. In this study, an alternative expression and purification protocol has been developed and validated to obtain overexpressed KCNE4 for

Indexed as

Escherichia coliPotassium Channels, Voltage-GatedCell MembraneCircular DichroismElectron Spin Resonance SpectroscopyHumansRecombinant ProteinsKCNE4 protein, humanPotassium Channels, Voltage-GatedRecombinant Proteins

Identifiers

PMID39780724
PMCPMC12662171

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.