Evidence map›Paper›PMID 39777515›Full record

ArticlePediatric surgery international2025

Vitamin A enhances PI3K/Akt signaling and mitigates enterocyte apoptosis in a mouse model of necrotizing enterocolitis.

Qian Su, Li Chen, Dong Liu, Yanzhen Xu, Jinxing Feng, Jialin Yu, Zhaoxia Zhang, Zhangbin Yu

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Article in Pediatric surgery international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Qian SuDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China.
Li ChenDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China.
Dong LiuDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China. liudong86755@hotmail.com.
Yanzhen XuDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China.
Jinxing FengDepartment of Neonatology, Shenzhen Children's Hospital, Shenzhen, 518020, Guangdong, China.
Jialin YuDepartment of Neonatology, Hospital of Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China.
Zhaoxia ZhangDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China.
Zhangbin YuDepartment of Neonatology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2021A1515110399Cultivation Project of Shenzhen People's Hospital SYJCYJ202007Shenzhen Scientific and Technological Foundation JCYJ20210324114207020
6 · The paper itself

Abstract

purposeThis study aims to elucidate the roles of the PI3K-Akt signaling pathway and enterocyte apoptosis in necrotizing enterocolitis (NEC) pathogenesis and investigate the impact of vitamin A intervention on these factors.

methodsWe employed an NEC mouse model and administered vitamin A treatment. Retinol levels in mouse blood were quantified using ELISA. Intestinal cell apoptosis in NEC mice was assessed via the TUNEL assay. We evaluated mRNA and protein expressions of Bcl-2, Bax, cytochrome C (CytoC), Caspase 3, and PI3K/Akt signaling pathway components using qPCR and western blotting.

resultsIn NEC models, PI3K, Akt, and Bcl-2 were downregulated, accompanied by upregulated Bax, CytoC, and Caspase 3 at both mRNA and protein levels. These molecular changes were associated with an increase in enterocyte apoptosis in the NEC models. Vitamin A supplementation increased PI3K, Akt, and Bcl-2 expression while decreasing Bax, CytoC, and Caspase 3 levels in the NEC models, resulting in reduced apoptosis.

conclusionVitamin A has the potential to mitigate enterocyte apoptosis in NEC by upregulating the PI3K/Akt signaling pathway and modulating apoptotic signals, providing new insights into the inhibitory effect of vitamin A on enterocyte apoptosis in NEC.

Indexed as

ApoptosisDisease Models, AnimalEnterocolitis, NecrotizingEnterocytesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionVitamin AAnimalsAnimals, NewbornBlotting, WesternEnzyme-Linked Immunosorbent AssayMiceMice, Inbred C57BLPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktVitamin AApoptosisNecrotizing enterocolitisPhosphoinositide 3-kinase/Akt signaling pathwayVitamin A

Identifiers

PMID39777515

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.