Cinnamaldehyde nanoemulsion decorated with rhamnolipid for inhibition of methicillin-resistant

Background: Methods: The formulation of cinnamaldehyde nanoemulsion (Cin-NE) combined with rhamnolipid (RHL) was developed by self-assembly, and the efficacies of this formulation in inhibiting Results: The particle size of the selected Cin-NE formulation was 13.66 ± 0.08 nm, and the Cin-RHL-NE formulation was 20.45 ± 0.25 nm. The selected Cin-RHL-NE formulation was stable at 4, 25, and 37°C. Furthermore, the Minimum Inhibitory Concentration (MIC) value of Cin-RHL-NE against MRSA was two-fold lower than drug solution. Confocal laser scanning microscopy (CLSM) revealed the superior efficacy of Cin-RHL-NE in eradicating MRSA biofilms while maintaining the Cin's inherent functional properties. The efficacy of Cin-RHL-NE in the mouse skin wound healing model was superior to other formulation. Conclusion: These findings highlight the potential of the formulation Cin-RHL-NE for eradicating biofilms, and effective in treating notoriously persistent bacterial infections. The Cin-RHL-NE can used as a dosage form of Cin application to bacterial biofilm associated infections.