Evidence map›Paper›PMID 39776097›Full record

ArticleJournal of fluorescence2025

Establishment of Time-Resolved Fluorescence Immunoassay for Thyrotropin Receptor Antibodies and Clinical Application.

Ting Shen, Fuzhou Yang, Jialong Wu, Yuan Qin, Xiumei Zhou, Xueqin Zhao, Bao Zhu, Biao Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ting Shen *College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China.
Fuzhou Yang *Nuclear Medicine, Ya 'an People's Hospital, Sichuan, 625000, P. R. China.
Jialong WuCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China.
Yuan QinCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China.
Xiumei ZhouCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China.
Xueqin ZhaoCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China.
Bao Zhu *Department of Nuclear Medicine, Wuxi Medical Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Nanjing Medical University, Wuxi, Jiangsu, 214023, P. R. China. zhubao_999@126.com.
Biao Huang *College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310017, China. jswxhb@163.com.

Funding

National Natural Science Foundation of China No. 82172336Scientific research project of Zhejiang Provincial Education Department Y202250731the Medical and Health Science and Technology Project of Zhejiang Province 2022KY265、2020KY753the Natural Science Foundation of Zhejiang Province LQ23H050005the "Pioneer" and "Leading Goose" R&D Program of Zhejiang No. 2022C03118, 2023C03075
6 · The paper itself

Abstract

Thyroid-stimulating hormone receptor antibody (TRAb) is a specific marker for Graves' disease (GD) and the measurement of which can improve the accuracy of GD diagnosis. Current detection methods utilize porcine-derived polyclonal-TRAb, which is unstable and is a source of significant inter-assay variability. This study aims to establish a time-resolved fluorescence immunoassay (TRFIA) method based on stable source of recombinant human TSHR and TRAb for the detection of serum TRAb. The neutralization inhibition method was used in this study. The specific binding of goat-anti-mouse IgG and chimeric construct Fc fragment human TSHR were immobilized on the microplate, and serum TRAb was detected by the competition between Eu

Indexed as

Graves DiseaseReceptors, ThyrotropinAnimalsFluoroimmunoassayHumansImmunoglobulin GTime FactorsImmunoglobulin GReceptors, ThyrotropinCompetitive methodsGrave’s diseaseSerum TRAbTRFIA

Identifiers

PMID39776097

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.