Evidence map›Paper›PMID 39775316›Full record

ArticleNeurosurgical review2025

AlkaPhos: a novel fluorescent probe as a potential point-of-care diagnostic tool to estimate recurrence risk of meningiomas.

Sina Hemmer, Xin Hui, Julia Draeger, Johannes Menges, Eva C Schwarz, Arne Wrede, Joachim Oertel, Lars Kaestner, Gregor Jung, Steffi Urbschat

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurosurgical review, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sina HemmerDepartment of Neurosurgery, Saarland University Medical Center, Homburg, Germany.
Xin HuiBiophysical Chemistry, Saarland University, Saarbrücken, Germany.
Julia DraegerBiophysical Chemistry, Saarland University, Saarbrücken, Germany.
Johannes MengesBiophysical Chemistry, Saarland University, Saarbrücken, Germany.
Eva C SchwarzBiophysics, Center for Integrative Physiology and Molecular Medicine, Saarland University, Homburg, Germany.
Arne WredeInstitute for Neuropathology, Saarland University Medical Center, Homburg, Germany.
Joachim OertelDepartment of Neurosurgery, Saarland University Medical Center, Homburg, Germany.
Lars KaestnerTheoretical Medicine and Biosciences, Saarland University, Homburg, Germany.
Gregor JungBiophysical Chemistry, Saarland University, Saarbrücken, Germany.
Steffi UrbschatDepartment of Neurosurgery, Saarland University Medical Center, Homburg, Germany. steffi.urbschat@uks.eu.ORCID http://orcid.org/0000-0003-2154-048X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Deletion of the short arm of chromosome 1 (1p) increases recurrence rates in meningiomas by up to 33%, regardless of tumor grade, correlating with absence of intracellular alkaline phosphatase enzyme activity. Current screening methods for 1p deletion like fluorescence in situ hybridization (FISH) and loss of heterozygosity (LOH) analysis are resource-intensive. This study evaluated AlkaPhos, a novel fluorescent probe, for detecting alkaline phosphatase in meningioma cells and compared findings with FISH, LOH, and histochemical analysis. AlkaPhos sensitivity in detecting alkaline phosphatase on BEN-MEN-1 cells and primary meningioma cultures was assessed via microscopic fluorescent ratio measurements. FISH and LOH were conducted on the same tumors to detect 1p deletions. Histochemical analysis served as a reference. AlkaPhos results were compared with FISH, LOH, and histochemical analysis. AlkaPhos effectively indicated alkaline phosphatase activity in BEN-MEN-1 cells and correctly identified 1p deletion in 8/14 primary meningioma cultures, matching FISH and LOH findings, respectively. AlkaPhos showed potential superiority over histochemical analysis in identifying tumors with 1p deletion and LOH of 1p. AlkaPhos bears potential as a future diagnostic tool for identifying alkaline phosphatase absence in meningiomas, indicative of 1p deletion. Further evaluation on a larger sample size is necessary for routine clinical application.

Indexed as

Alkaline PhosphataseFluorescent DyesMeningeal NeoplasmsMeningiomaMolecular Probe TechniquesNeoplasm Recurrence, LocalPoint-of-Care TestingCell Line, TumorChromosome DeletionChromosomes, Human, Pair 1HumansIn Situ Hybridization, FluorescencePrimary Cell CultureRisk AssessmentAlkaline PhosphataseFluorescent DyesAlkaline phosphatase detectionDeletion of chromosome 1pFluorescence microscopyMeningioma recurrencePrimary meningioma cell cultures

Identifiers

PMID39775316
PMCPMC11706915

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.