Evidence map›Paper›PMID 39774652›Full record

ArticleScientific reports2025

Elucidation of peptide screen for targeted identification of Yersinia pestis by nano-liquid chromatography tandem mass spectrometry.

Priya Rani, Syed Imteyaz Alam, Sandeep Singh, Subodh Kumar

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Priya RaniMicrobiology Division, Defence Research and Developmental Establishment, Jhansi Road, Gwalior, 474002, India.
Syed Imteyaz AlamBiotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474002, India.
Sandeep SinghMicrobiology Division, Defence Research and Developmental Establishment, Jhansi Road, Gwalior, 474002, India.
Subodh KumarMicrobiology Division, Defence Research and Developmental Establishment, Jhansi Road, Gwalior, 474002, India. subodh.drde@gov.in.ORCID 0000-0002-8764-3361

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Yersinia pestis, a Gram-negative bacterium is the causative agent of the fatal communicable disease plague. The disease had a profound impact on human history. Plague bacteria are usually transmitted to humans through the bite of an infected rat flea. Earlier studies have indicated that Y. pestis can survive in environmental matrices e.g. water and soil. This study aimed to generate a peptide-based screen for identification of Y. pestis particularly from environmental matrices. We employed a shotgun proteomic approach using nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) to discover Y. pestis-specific peptides. The pure cultures of Y. pestis and related species were grown, their proteome were delineated and analyzed by in silico tools to discover 61 Y. pestis specific peptides. Additionally, 148 peptides were discovered from proteins of Y. pestis-specific plasmids and chromosomal-associated virulence markers. To validate this screen of 209 peptides, various concentrations of Y. pestis (ranging from 1.3 × 10

Indexed as

Bacterial ProteinsPeptidesTandem Mass SpectrometryYersinia pestisChromatography, LiquidHumansPlagueProteomeProteomicsSoil MicrobiologyBacterial ProteinsPeptidesProteomenLC-MS/MSPeptide-based screenYersinia pestis

Identifiers

PMID39774652
PMCPMC11707332

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.