Evidence map›Paper›PMID 39773298›Full record

ArticleParasites & vectors2025

Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs.

Rafaela Lira Nogueira de Luna, Kamila Gaudêncio da Silva Sales, Lucas Lisboa Nunes Bonifácio, Luciana Aguiar Figueredo, Thomas R Shelite, Fábio Dos Santos Nogueira, Domenico Otranto, Filipe Dantas-Torres

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Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Rafaela Lira Nogueira de LunaAggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil.
Kamila Gaudêncio da Silva SalesAggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil.
Lucas Lisboa Nunes BonifácioAggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil.
Luciana Aguiar FigueredoAggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil.
Thomas R SheliteDepartment of Biosafety, University of Texas Medical Branch, Galveston, USA.
Fábio Dos Santos NogueiraAndradina Educational Foundation, Andradina, Brazil.
Domenico OtrantoDepartment of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy.
Filipe Dantas-TorresAggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil. dantastorrespv@gmail.com.

Funding

Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco APQ-0548- 2.13/19
6 · The paper itself

Abstract

backgroundWe standardized two recombinase polymerase amplification (RPA) assays coupled with lateral flow (LF) strips for the detection of Leishmania braziliensis and Leishmania infantum kinetoplast DNA (kDNA).

methodsThe RPA-LF assays were tested at different temperatures and reaction times, using DNA from cultured L. braziliensis and L. infantum. The L. infantum RPA-LF was also tested using clinical samples (bone marrow and skin) from infected and uninfected dogs.

resultsThe detection limits (analytical sensitivity) of the assays were 0.04 pg/μl and 0.04 ng/μl for L. braziliensis and L. infantum kDNA, respectively. Using clinical samples, the L. infantum RPA-LF successfully detected the parasite kDNA in bone marrow (21/30; 70.0%) and skin samples (23/30, 76.6%) from naturally infected dogs. We found an almost perfect agreement (kappa = 0.807) between RPA-LF for L. infantum and our reference quantitative real-time polymerase chain reaction (qPCR), considering clinical samples with a quantification cycle (C

conclusionsThe RPA-LF assays developed here may be promising diagnostic tools for point-of-care diagnosis of L. infantum and L. braziliensis infection in dogs, particularly in remote rural areas lacking laboratory infrastructure.

Indexed as

DNA, KinetoplastDog DiseasesLeishmania braziliensisLeishmania infantumMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSensitivity and SpecificityAnimalsDNA, ProtozoanDogsLeishmaniasis, CutaneousLeishmaniasis, VisceralReal-Time Polymerase Chain ReactionSkinDNA, KinetoplastDNA, ProtozoanIsothermal amplificationLeishmaniasisMolecular diagnosisPoint-of-careRecombinase

Identifiers

PMID39773298
PMCPMC11705694

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.