Evidence map›Paper›PMID 39772168›Full record

ArticleViruses2024

Development of a Multiplex RT-PCR Detection for Six Viruses Infecting Strawberry.

Yong Wang, Xiangguo Zeng, Guilin Xiao, Dongmei Zhang, Xin Wen, Xinxin Zhou, Zexian Wang, Jiangli Deng, Yongchao Han

Abstract read
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yong WangInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.ORCID 0000-0002-9450-0748
Xiangguo ZengInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Guilin XiaoInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Dongmei ZhangInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Xin WenInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Xinxin ZhouInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Zexian WangInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Jiangli DengInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Yongchao HanInstitute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.ORCID 0000-0001-9985-9912

Funding

Agricultural Science and Technology Innovation Center Program of Hubei Province 2021-620-000-001-008Hubei Province's Technological Innovation Plan Project for the year 2024 NYNC202400286Key Research Program of Hubei Province 2023BBB041; 2023BBB133Open Bidding for Selecting the Best Candidates Program of Wuhan East Lake High-tech Zone 2022KJB134
6 · The paper itself

Abstract

Strawberry viruses are significant pathogenic agents in strawberry. The development and application of efficient virus detection technology can effectively reduce the economic losses incurred by virus diseases for strawberry cultivators. In order to rapidly identify strawberry virus species and prevent the spread of virus disease, a multiplex reverse transcription polymerase chain reaction system was established for the simultaneous detection and identification of strawberry mild yellow edge virus (SMYEV), strawberry vein banding virus (SVBV), strawberry mottle virus (SMoV), strawberry polerovirus 1 (SPV-1), strawberry pallidosis-associated virus (SPaV), and strawberry crinivirus 4 (SCrV-4). In this study, six pairs of specific primers were designed on the conserved genomic regions of these viruses. The primer concentration, annealing temperature, and amplification cycle number of the reaction system were optimized. Subsequent sensitivity testing and application of the optimized detection system were carried out. The results indicate the establishment of an efficient detection system for strawberry viruses. The optimal reaction can detect the six viruses at the same time, which provides technical support for the early prevention and treatment of strawberry virus diseases.

Indexed as

FragariaMultiplex Polymerase Chain ReactionPlant DiseasesPlant VirusesDNA PrimersReverse Transcriptase Polymerase Chain ReactionRNA, ViralSensitivity and SpecificityDNA PrimersRNA, Viralmultiplex RT-PCRSCrV-4SMoVSMYEVSPaVSPV-1strawberrySVBV

Identifiers

PMID39772168
PMCPMC11680342

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.