Evidence map›Paper›PMID 39772141›Full record

ArticleViruses2024

4D-DIA-Based Quantitative Proteomic Analysis Reveals the Involvement of TRPV2 Protein in Duck Tembusu Virus Replication.

Jimin Chen, Fan Yang, Lianjie Lai, Huihuang Li, Chengfu Pan, Xinguo Bao, Weimin Lin, Ruiyi Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jimin ChenCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fan YangCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Lianjie LaiCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Huihuang LiCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Chengfu PanCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Xinguo BaoCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Weimin LinCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Ruiyi LinCollege of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.ORCID 0000-0003-3255-5900

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV), a novel positive-sense RNA virus, has caused significant economic losses in the poultry industry of Eastern and Southeast Asia since its outbreak in 2010. Furthermore, the rapid transmission and potential zoonotic nature of DTMUV pose a threat to public health safety. In this study, a 4D-DIA quantitative proteomics approach was employed to identify differentially expressed cellular proteins in DTMUV-infected DF-1 cells, which are routinely used for virus isolation and identification for DTMUV, as well as the development of vaccines against other poultry viruses. One hundred fifty-seven differentially expressed cellular proteins were identified, including 84 upregulated and 73 downregulated proteins at 48 h post-infection, among which CXCL8, DDX3X, and TRPV2 may play crucial roles in viral propagation. Notably, for the upregulated protein TRPV2, the DTMUV replication was inhibited in TRPV2-low-expressing DF-1 cells. In summary, our research represents the application of 4D-DIA quantitative proteomics to analyze the proteomic landscape of DTMUV-infected poultry cells. These findings may provide valuable insights into understanding the interaction mechanism between DTMUV and poultry cells, as well as the identification of disease-resistant host factors in poultry breeding research.

Indexed as

DucksFlavivirusPoultry DiseasesProteomicsVirus ReplicationAnimalsCell LineFlavivirus InfectionsProteomeTRPV Cation ChannelsProteomeTRPV Cation ChannelsDF-1 cellsduck Tembusu virusquantitative proteomicsTRPV2

Identifiers

PMID39772141
PMCPMC11680370

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.