Evidence map›Paper›PMID 39770918›Full record

Trial reportNutrients2024

Investigating the Impact of Glycogen-Depleting Exercise Combined with Prolonged Fasting on Autophagy and Cellular Health in Humans: A Randomised Controlled Crossover Trial.

Andrius Masedunskas, Isabella de Ciutiis, Leanne K Hein, Anjie Ge, Yvonne X Kong, Miao Qi, Drishya Mainali, Lara Rogerson-Wood, Cynthia M Kroeger, Yvonne A Aguirre Candia and 8 more

Abstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Nutrients, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Links Between Autophagy and Healthy Aging.Journal of molecular biology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Andrius MasedunskasCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Isabella de CiutiisCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Leanne K HeinLysosomal Health in Ageing, Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA 5000, Australia.
Anjie GeCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-1027-2793
Yvonne X KongCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Miao QiCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Drishya MainaliCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Lara Rogerson-WoodCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Cynthia M KroegerCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-5828-4283
Yvonne A Aguirre CandiaCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Maria L CagigasCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0001-5464-2349
Tian WangCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0001-7217-4200
David HutchinsonDepartment of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2006, Australia.
Angelo SabagCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-0195-7029
Freda H PassamCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0003-4584-2044
Laura PiccioCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
Timothy J SargeantLysosomal Health in Ageing, Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA 5000, Australia.ORCID 0000-0003-1254-4390
Luigi FontanaCharles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.

Funding

National Health and Medical Research Council APP1177797
6 · The paper itself

Abstract

importanceAlthough prolonged fasting has become increasingly popular, the favourable biological adaptations and possible adverse effects in humans have yet to be fully elucidated.

objectiveTo investigate the effects of a three-day water-only fasting, with or without exercise-induced glycogen depletion, on autophagy activation and the molecular pathways involved in cellular damage accumulation and repair in healthy humans.

designA randomised, single-centre, two-period, two-sequence crossover trial. The primary outcome is autophagic activity, assessed as flux in peripheral blood mononuclear cells (PBMCs) measured in the context of whole blood. Secondary outcomes include changes in body composition, heart rate variability, endothelial function, and genomic, epigenomic, metabolomic, proteomic, and metagenomic adaptations to fasting in plasma, platelets, urine, stools, and PBMCs. Detailed profiling of circulating immune cell populations and their functional states will be assessed by flow cytometry.

settingAll clinical investigations will be undertaken at the Charles Perkins Centre Royal Prince Alfred Hospital clinic, University of Sydney, Australia.

participantsTwenty-four individuals aged 18 to 70 years, with a BMI of 20-40 kg/m DISCUSSION: While autophagic flux induction through fasting has garnered interest, there is a notable lack of human studies on this topic. This trial aims to provide the most detailed and integrated analysis of how three days of prolonged water-only fasting, combined with glycogen-depleting exercise, affects autophagy activation and other crucial metabolic and molecular pathways linked to cellular, metabolic, and immune health. Insights from this study may pave the way for safe and effective strategies to induce autophagy, offering potential preventive interventions for a range of chronic conditions.

Indexed as

AutophagyCross-Over StudiesExerciseFastingGlycogenAdolescentAdultAgedFemaleHumansLeukocytes, MononuclearMaleMiddle AgedYoung AdultGlycogenautophagyexercise trainingfastingketone bodiesmetabolism

Identifiers

PMID39770918
PMCPMC11677747

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.