Evidence map›Paper›PMID 39770370›Full record

ArticlePathogens (Basel, Switzerland)2024

BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting

Sonoko Watanuki, Aronggaowa Bao, Etsuko Saitou, Kazuyuki Shoji, Masaki Izawa, Mitsuaki Okami, Yasunobu Matsumoto, Yoko Aida

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. First Identification ofPathogens (Basel, Switzerland) · 2025
    Article
  2. Polymorphism ofPathogens (Basel, Switzerland) · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sonoko WatanukiLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0006-8408-2622
Aronggaowa BaoLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0009-0001-2424-4937
Etsuko SaitouHyogo Prefectural Awaji Meat Inspection Center, 49-18 Shitoorinagata, Minamiawaji 656-0152, Japan.
Kazuyuki ShojiMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Masaki IzawaMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Mitsuaki OkamiMolecular Diagnosis Division, Nippon Gene Co., Ltd., 2-8-16 Toiya-machi, Toyama 930-0834, Japan.
Yasunobu MatsumotoLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoko AidaLaboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.ORCID 0000-0001-7400-3587

Funding

a grant from the Livestock Promotional Subsidy from the Japan Racing Association (JRA)a Grant-in-Aid for JSPS Fellow from the Japan Society for the Promotion of Science (JSPS) 23KJ0412collaborative research fund from Nippon Gene Co., Ltd.
6 · The paper itself

Abstract

The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the "Liquid Dual-CoCoMo assay", that uses the coordination of common motif (CoCoMo) degenerate primers. This method can detect two genes simultaneously using a FAM-labeled minor groove binder (MGB) probe for the BLV long terminal repeat (LTR) region and a VIC-labeled MGB probe for the

Indexed as

Enzootic Bovine LeukosisLeukemia Virus, BovineReal-Time Polymerase Chain ReactionSensitivity and SpecificityTerminal Repeat SequencesViral LoadAnimalsCattleMultiplex Polymerase Chain ReactionProvirusesbovine leukemia virus (BLV)commercial kitduplexlong terminal repeat (LTR)pol geneproviral load (PVL)quantitative real-time PCR (qPCR)

Identifiers

PMID39770370
PMCPMC11677995

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.