Variable Assembly and Procapsid Binding of Bacteriophage P22 Terminase Subunits in Solution.

Julia Elise Cabral, Yanfei Qiu, Albert J R Heck, Reginald McNulty

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Dissecting the dsDNA viral packaging machinery: structural evolution and potential for therapeutics.Journal of virology · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Julia Elise CabralLaboratory of Macromolecular Structure, Department of Molecular Biology and Biochemistry, University of California Irvine, Steinhaus Hall, Irvine, CA 92697-3900, USA.ORCID 0000-0001-6072-6362

Yanfei QiuLaboratory of Macromolecular Structure, Department of Molecular Biology and Biochemistry, University of California Irvine, Steinhaus Hall, Irvine, CA 92697-3900, USA.ORCID 0009-0009-2036-8586

Albert J R HeckBiomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Reginald McNultyLaboratory of Macromolecular Structure, Department of Molecular Biology and Biochemistry, University of California Irvine, Steinhaus Hall, Irvine, CA 92697-3900, USA.ORCID 0000-0003-2101-1377

Funding

Immunology Research Training GrantT32AI177324 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI Eric Pearlman · 2023 to 2026

$735k

Macromolecular assemblies of transcription factors initiated by pathogen infectionK22AI139444 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI MCNULTY, REGINALD · 2019 to 2021

$297k

NIAID NIH HHS K22 AI139444NIAID NIH HHS K22AI139444NIAID NIH HHS L30 AI140191NIAID NIH HHS T32 AI177324

6 · The paper itself

Abstract

Concatemeric viral DNA is packaged into bacteriophage P22 procapsids via a headful packaging mechanism mediated by a molecular machine consisting of small (gp3) and large (gp2) terminase subunits. Although a negative stain reconstruction exists for the terminase holoenzyme, it is not clear how this complex binds the dodecameric portal protein located at a 5-fold mismatch vertex. Herein, we describe new assemblies for the holoenzyme. Both native mass spectrometry and transmission electron microscopy reveal that the P22 terminase complex adopts three main assemblies, which include a nonameric S-terminase bound to two L-terminase 1(gp3)

Indexed as

Bacteriophage P22EndodeoxyribonucleasesCapsidDNA, ViralMass SpectrometryMicroscopy, Electron, TransmissionProtein BindingProtein SubunitsVirus AssemblyDNA, ViralEndodeoxyribonucleasesProtein Subunitsterminasebacteriophage P22electron microscopylarge terminaseSalmonella virussmall terminaseviral genome-packaging motor

Identifiers

PMID39770326

DOI10.3390/pathogens13121066

PMCPMC11728703

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.