ArticleLife (Basel, Switzerland)2024
Effects of Sequential Antimicrobial Phases on Root Canal Microbiome Dynamics in Two-Visit Treatment of Primary Apical Periodontitis: A Longitudinal Experimental Study.
Article in Life (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- Microbial dynamics in primary and refractory apical periodontitis: Next-generation 16S rRNA sequencing reveals selective shifts and reduced diversity.Journal of conservative dentistry and endodontics · 2026Article
- Quantitative Evaluation of the Inhibitory Effects of Commercially Available Probiotics on Dual-Species Biofilms in Root Canals: A qPCR-Based Short-Term In Vitro Study.Antibiotics (Basel, Switzerland) · 2026Article
- The conundrum of current endodontic disinfection strategies in microbial load reduction: a scoping review.BMC oral health · 2026Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundEffective management of primary apical periodontitis depends on understanding the dynamic interactions within the root canal microbiome. This study aimed to investigate the effect of sequential antimicrobial phases on the root canal microbiome during a two-visit treatment approach, with a focus on calcium hydroxide medication.
methodsSamples were collected from three teeth across four treatment phases: initial infection (S1), after chemomechanical preparation (S2), after intracanal medication (S3), and after a final flush (S4). DNA was extracted, and the V3-V4 regions of the 16S rRNA gene were sequenced using Illumina MiSeq. Sequencing data were analyzed with QIIME 2, and differentially abundant taxa were identified using linear discriminant analysis effect size (LEfSe).
resultsWhile microbial community composition did not differ significantly between phases, the
conclusionsAlthough calcium hydroxide treatment did not induce statistically significant changes in overall root canal microbial composition, trends such as a reduction in the
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