Evidence map›Paper›PMID 39768023›Full record

ArticleBioengineering (Basel, Switzerland)2024

TGF-β3 Restrains Osteoclastic Resorption Through Autophagy.

Hui Liao, Yiqin Pan, Yiming Liu, Yuxiao Li, Shiyi Huang, Shan Ding, Qi Xiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hui LiaoState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Yiqin PanState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Yiming LiuState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Yuxiao LiState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Shiyi HuangState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Shan DingState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.
Qi XiangState Key Laboratory of Bioactive Molecules and Drug Gability Assessment, Jinan University, No. 855 East Xingye Avenue, Guangzhou 510632, China.ORCID 0000-0001-9790-5823

Funding

Guangzhou Research and Development Plan in Key Fields, China 202103030003National College Students' innovation and entrepreneurship training program 202310559101National Key Research and Development Program of China 2022YFC2403102Natural Science Foundation of Guangdong Province, China 2021A1515012480R&D Plan Project in Key Fields of Guangdong Province, China 2022B1111080007
6 · The paper itself

Abstract

While TGF-β3 promoted defect healing in a primate baboon skull defect model and patients, it remains unclear whether TGF-β3 affects the formation of osteoclasts and bone resorption between osteogenesis and osteolysis. Analysis of the full transcriptome of hPDLSCs (human periodontal ligament stem cells) revealed that the expression of RANKL was significantly up-regulated after TGF-β3 treatment during osteogenesis, which suggests its involvement in clock-controlled autophagy in bone metabolism. TRAP staining and bone resorption lacunae were used to assess the osteoclasts formed from RANKL-induced differentiated BMMs. During osteoclast differentiation, the characteristics of autophagy regulated by TGF-β3 were observed in BMMs through MDC staining, transmission electron microscopy, and LC3 immunofluorescence. The expression of related genes and proteins were detected on the sixth day in mCherry-EGFP-LC3B lentivirus-transfected BMMs using RT-qPCR and WB. Finally, a trans-well co-culture system was used to evaluate the effects of osteogenic differentiated hPDLSCs treated with TGF-β3 on the osteoclastic differentiation of BMMs. The results showed 10 ng/mL of TGF-β3 significantly suppressed osteoclastic differentiation and bone resorption in BMMs (

Indexed as

autophagybone injury repairbone marrow macrophagesosteoclast differentiationtransforming growth factor 3

Identifiers

PMID39768023
PMCPMC11673033

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.