ArticleBioengineering (Basel, Switzerland)2024
Effects of Extreme Temperature on Human Bronchial Epithelial Cells in 3D Printed Samples.
Article in Bioengineering (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Feasible Regions of Nozzle Temperature, Extrusion Pressure, and Printing Speed in Extrusion-Based Printing Using a Sodium Alginate-Carboxymethylcellulose-Collagen I Bioink.Biomimetics (Basel, Switzerland) · 2026Article
- Prospects and Limitations of Bioprinting in Studying Human Cells' Responses to Extreme Environments.Bioengineering (Basel, Switzerland) · 2026Article
- Effects of Extrusion Pressure During 3D Printing on Viability of Human Bronchial Epithelial Cells in 3D Printed Samples.Biomimetics (Basel, Switzerland) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
This paper reports an experimental study on the effects of extreme temperature on human bronchial epithelial (HBE) cells encapsulated in 3D printed samples. Well plates of the 3D printed samples were exposed to three levels of temperature (37 °C, 45 °C, and 55 °C, respectively) for a duration of 10 min. Cells' responses, specifically cell viability and oxidative stress, were quantified using Hoechst 33342, Sytox, and Mitosox stains, with intensity measurements obtained via a plate reader. In addition, cell viability was assessed through microscopic imaging of the 3D printed samples. Experimental results demonstrated that the temperature increase from 37 °C to 55 °C significantly reduced nuclear integrity as observed through Hoechst 33342 intensity, while increased Sytox intensity reflected a higher degree of cell death. Furthermore, cells exposed to 45 °C and 55 °C exhibited decreased cell viability and elevated mitochondrial oxidative stress. These findings offer valuable insights into the effects of extreme temperature on HBE cells, establishing a foundation for future research into how respiratory tissues respond to thermal stress. This research can potentially advance the knowledge regarding effects of heat exposure on the respiratory system.
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Registered trials
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