Evidence map›Paper›PMID 39767659›Full record

ArticleBiomedicines2024

DENV-1 Infection of Macrophages Induces Pyroptosis and Causes Changes in MicroRNA Expression Profiles.

Qinyi Zhang, Sicong Yu, Zhangnv Yang, Xingxing Wang, Jianhua Li, Lingxuan Su, Huijun Zhang, Xiuyu Lou, Haiyan Mao, Yi Sun and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedicines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Qinyi ZhangZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Sicong YuZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Zhangnv YangZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Xingxing WangZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Jianhua LiZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.ORCID 0000-0002-9216-4699
Lingxuan SuZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Huijun ZhangInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, Beijing 100021, China.ORCID 0009-0008-8884-625X
Xiuyu LouZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Haiyan MaoZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Yi SunZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Lei FangDepartment of Critical Care Medicine, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.ORCID 0000-0002-9916-5782
Hao YanZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Yanjun ZhangZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.

Funding

Medical Science and Technology Program of Zhejiang Province 2024KY888, 2024KY907, 2021KY620
6 · The paper itself

Abstract

backgroundDengue virus (DENV) is the most widespread mosquito-borne virus, which can cause dengue fever with mild symptoms, or progress to fatal dengue hemorrhagic fever and dengue shock syndrome. As the main target cells of DENV, macrophages are responsible for the innate immune response against the virus.

methodsIn this study, we investigated the role of pyroptosis in the pathogenic mechanism of dengue fever by examining the level of pyroptosis in DENV-1-infected macrophages and further screened differentially expressed microRNAs by high-throughput sequencing to predict microRNAs that could affect the pyroptosis of the macrophage.

resultsMacrophages infected with DENV-1 were induced with decreased cell viability, decreased release of lactate dehydrogenase and IL-1β, activation of NLRP3 inflammasome and caspase-1, cleavage of GSDMD to produce an N-terminal fragment bound to cell membrane, and finally induced macrophage pyroptosis. MicroRNA expression profiles were obtained by sequencing macrophages from all periods of DENV-1 infection and comparing with the negative control. Sixty-three microRNAs differentially expressed in both the early and later stages of infection were also identified. In particular, miR-223-3p, miR-148a-3p, miR-125a-5p, miR-146a-5p and miR-34a-5p were recognized as small molecules that may be involved in the regulation of inflammation.

conclusionsIn summary, this study aimed to understand the pathogenic mechanism of DENV through relevant molecular mechanisms and provide new targets for dengue-specific therapy.

Indexed as

dengue virusmacrophagesmicroRNApyroptosis

Identifiers

PMID39767659
PMCPMC11673035

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.