Evidence map›Paper›PMID 39766850›Full record

ArticleGenes2024

Kir4.1 and Aqp4 Contribution to Schisis Cystic Water Accumulation and Clearance in the Rs1 Exon-1 Del XLRS Rat Model.

Zeljka Smit-McBride, Ning Sun, Serafina Thomas, In Hwan Cho, Robin G Stricklin, Paul A Sieving

Abstract read
In one paragraph

Article in Genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zeljka Smit-McBrideDepartment of Ophthalmology, Eye Center, UC Davis School of Medicine, University of California Davis, Sacramento, CA 95817, USA.ORCID 0000-0003-0275-8088
Ning SunDepartment of Cell Biology & Human Anatomy, University of California Davis, Davis, CA 95616, USA.
Serafina ThomasDepartment of Cell Biology & Human Anatomy, University of California Davis, Davis, CA 95616, USA.
In Hwan ChoDepartment of Ophthalmology, Eye Center, UC Davis School of Medicine, University of California Davis, Sacramento, CA 95817, USA.ORCID 0000-0002-2384-1502
Robin G StricklinDepartment of Cell Biology & Human Anatomy, University of California Davis, Davis, CA 95616, USA.
Paul A SievingDepartment of Ophthalmology, Eye Center, UC Davis School of Medicine, University of California Davis, Sacramento, CA 95817, USA.ORCID 0000-0002-2918-577X

Funding

VISION RESEARCH CORE GRANTP30EY012576 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI W MARTIN USREY · 1999 to 2026
$18.9M
Intramural Research Program of the NIH, NEI DC900065NEI Core Facilities grant to UCD P30-EY012576NEI NIH HHS P30 EY012576UC Davis Medical School Dean's Fund for laboratory startup to PAS n/a
6 · The paper itself

Abstract

BACKGROUND/

objectiveThe Rs1 exon-1-del rat (Rs1KO) XLRS model shows normal retinal development until postnatal day 12 (P12) when small cystic spaces start to form in the inner nuclear layer. These spaces enlarge rapidly, peak at P15, and then collapse by P19.

methodsWe explored the possible involvement of Kir4.1 and Aqp4, the principal retina channels for water movement and homeostasis, along with Muller glia cells (MGCs), using semi-quantitative fluorescent immunohistochemistry at P7, P9, P12, and P30, in Rs1KO and WT littermates.

resultsKir4.1 expression was reduced in Rs1KO retinas at all the early time points-P7, P9, and P12-as the schisis cavities began to form; downregulation would reduce water egress from the retina. Aqp4 was upregulated at P30 in Rs1KO retinas during schisis cavity closure but not as cavities formed at P12. When examined by GFAP expression, MGCs were not activated at the preschisis P12 age but showed considerable GFAP expression at P30 following retinal cystic structural damage at P15, indicating that MGCs were activated during the period of retina water removal and cavity closure.

conclusionsThe study results implicate the downregulation of Kir4.1 in schisis formation and a role for both Kir4.1 and Aqp4 upregulation in subsequent schisis closure.

Indexed as

Aquaporin 4Potassium Channels, Inwardly RectifyingRetinaAnimalsDisease Models, AnimalEpendymoglial CellsExonsKcnj10 ChannelRatsWaterAqp4 protein, ratAquaporin 4Kcnj10 ChannelPotassium Channels, Inwardly RectifyingWaterAqp4aquaporin4deep capillary plexusKir4.1MGCMuller glia cellsrat retina disease modelretina developmentretinoschisinRS1X-linked retinoschisisXLRS

Identifiers

PMID39766850
PMCPMC11675908

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.