ArticleBiomolecules2024
Characterization of the Interaction of Human γS Crystallin with Metal Ions and Its Effect on Protein Aggregation.
Article in Biomolecules, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Curcumin Nanoemulsion: Characterization and Effect on Cataracts in an In Vivo Animal Model and Ex Vivo Human Model.Biomolecules · 2026Article
- Metal ions in aging and ocular diseases: biology, pathophysiology, and therapeutic strategies.npj aging · 2026Review
- Case report of a CRYGS gene mutation in a patient with congenital cataracts and secondary glaucoma.American journal of translational research · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cataracts are diseases characterized by the opacity of the ocular lens and the subsequent deterioration of vision. Metal ions are one of the factors that have been reported to induce crystallin aggregation. For HγS crystallin, several equivalent ratios of Cu(II) promote protein aggregation. However, reports on zinc are contradictory. To characterize the process of metal ion binding and subsequent HγS crystallin aggregation, we performed dynamic light scattering, turbidimetry, isothermal titration calorimetry, fluorescence, and nuclear magnetic resonance experiments. The data show that both metal ions have multiple binding sites and promote aggregation. Zinc interacts mainly with the N-terminal domain, inducing small conformational changes, while copper interacts with both domains and induces unfolding, exposing the tryptophan residues to the solvent. Our work provides insight into the mechanisms of metal-induced aggregation at one of the lowest doses that appreciably promote aggregation over time.
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