Evidence map›Paper›PMID 39766258›Full record

ArticleBiomolecules2024

Fast and Simple Protocol for N-Glycome Analysis of Human Blood Plasma Proteome.

Denis E Maslov, Anna N Timoshchuk, Alexander A Bondar, Maxim P Golubev, Anna G Soplenkova, Maja Hanic, Sodbo Z Sharapov, Olga N Leonova, Yurii S Aulchenko, Tatiana S Golubeva

Abstract read
In one paragraph

Article in Biomolecules, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Denis E MaslovMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.ORCID 0000-0003-2806-5933
Anna N TimoshchukMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.ORCID 0000-0001-8529-4498
Alexander A BondarInstitute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk 630090, Russia.ORCID 0000-0001-9181-0487
Maxim P GolubevMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.
Anna G SoplenkovaMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.ORCID 0000-0003-0703-146X
Maja HanicGenos Glycoscience Research Laboratory, 10000 Zagreb, Croatia.ORCID 0000-0001-6152-2054
Sodbo Z SharapovMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.ORCID 0000-0003-0279-4900
Olga N LeonovaPriorov Central Institute of Traumatology and Orthopedic, Moscow 127299, Russia.ORCID 0000-0002-9916-3947
Yurii S AulchenkoMSU Institute for Artificial Intelligence, Lomonosov Moscow State University, Moscow 119192, Russia.ORCID 0000-0002-7899-1575
Tatiana S GolubevaInstitute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia.ORCID 0000-0002-4400-0665

Funding

Research Program at the MSU Institute for Artificial Intelligence
6 · The paper itself

Abstract

N-glycome analysis of individual proteins and tissues is crucial for fundamental and applied biomedical research and medical diagnosis and plays an important role in the evaluation of the quality of biopharmaceutical and biotechnological products. The interest in this research area continues to grow annually, thereby increasing the demand for the high-throughput profiling of human blood plasma N-glycome. In response to this need, we have developed an optimized, simple, and rapid protocol for the N-glycome profiling of human plasma proteins. This protocol encompasses the entire analysis cycle, from plasma isolation to N-glycan spectrum quantification. While the proposed method may have lower efficiency compared to already published high-throughput methods, its adaptability makes it suitable for implementation in virtually any molecular biological laboratory.

Indexed as

Blood ProteinsPolysaccharidesProteomeGlycomicsGlycoproteinsHumansBlood ProteinsGlycoproteinsPolysaccharidesProteomeAPTS labelinghuman blood plasmaN-glycansN-glycosylationpost-translational modificationsxCGE-LIF analysis

Identifiers

PMID39766258
PMCPMC11673551

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.