Evidence map›Paper›PMID 39765563›Full record

ArticleAnimals : an open access journal from MDPI2024

Genome-Wide In Silico Analysis of Microsatellite Loci in Rabbits.

Hosam M Safaa, Mostafa Helal, Seif Yasser, Zahra Raafat, Habiba Ayman, Hasnaa Mostafa, Milena Bozhilova-Sakova, Dalia A A Elsayed

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In one paragraph

Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Hosam M SafaaDepartment of Biology, College of Science, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia.ORCID 0000-0002-9192-6313
Mostafa HelalDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.ORCID 0000-0002-8005-9023
Seif YasserBiotechnology Program, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.ORCID 0009-0003-3479-4291
Zahra RaafatBiotechnology Program, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Habiba AymanBiotechnology Program, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Hasnaa MostafaBiotechnology Program, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Milena Bozhilova-SakovaAgricultural Academy, Institute of Animal Science, 2232 Kostinbrod, Bulgaria.ORCID 0000-0002-7637-1217
Dalia A A ElsayedDepartment of Poultry Breeding, Animal Production Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt.ORCID 0000-0001-5995-4253

Funding

University of Bisha Fast-Track Research Support Program
6 · The paper itself

Abstract

This study aimed to characterize microsatellites in the rabbit genome using an in silico approach and to develop and validate microsatellite markers. Blood samples were collected from 15 Baladi rabbits and 18 New Zealand White (NZW rabbits). The GMATA software was used to define SSRs in the extracted sequences. Twelve primer pairs were used to validate the loci identified and the primers developed. The total number of the detected microsatellite loci overall chromosomes was 1,136,253. The di-nucleotide microsatellite repeats dominated and exceeded 88% of the detected microsatellites in all chromosomes. There were no microsatellites detected in mitochondrial DNA. The highest relative microsatellite abundance was obtained for chromosome 19, followed by 13 and 6. The highest estimated SSR density was obtained for chromosome 14, and the lowest was for mitochondrial DNA, followed by chromosome 13. The polymorphism was 81.63% and 75.51% for Baladi and NZW rabbits, respectively. The number of detected alleles ranged between two and seven alleles/loci, and polymorphic information content was from 35% to 71%. The AMOVA analysis showed that the total variance of all levels of population structure was 15.734. The results definitely confirmed higher genetic diversity in Baladi compared with NZW rabbits.

Indexed as

genetic diversityminingpopulation structurerabbitsSSRsverification

Identifiers

PMID39765563
PMCPMC11672705

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.