Evidence map›Paper›PMID 39763895›Full record

ArticlebioRxiv : the preprint server for biology2024

Non-Canonical Cytochrome P450 Enzymes in Nature.

Andy K L Nguy, Kendra A Ireland, Chase M Kayrouz, Juan Carlos Cáceres, Brandon L Greene, Katherine M Davis, Mohammad R Seyedsayamdost

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andy K L NguyDepartment of Chemistry, Princeton University, Princeton, NJ, USA.
Kendra A IrelandDepartment of Chemistry, Emory University, Atlanta, GA, USA.
Chase M KayrouzDepartment of Chemistry, Princeton University, Princeton, NJ, USA.
Juan Carlos CáceresInterdepartmental Program in Quantitative Biosciences, University of California Santa Barbara, Santa Barbara, CA, USA.
Brandon L GreeneInterdepartmental Program in Quantitative Biosciences, University of California Santa Barbara, Santa Barbara, CA, USA.
Katherine M DavisDepartment of Chemistry, Emory University, Atlanta, GA, USA.
Mohammad R SeyedsayamdostDepartment of Chemistry, Princeton University, Princeton, NJ, USA.

Funding

Unraveling the dynamics that enable unusual heme enzyme reactivityR35GM147557 · NIGMS · EMORY UNIVERSITY · PI Katherine Marie Davis · 2022 to 2026
$1.9M
From Genes to Molecules and Back: Expanding the Chemical Space of Microbial and Microbiome Natural ProductsR35GM152049 · NIGMS · PRINCETON UNIVERSITY · PI Mohammad R Seyedsayamdost · 2024 to 2026
$1.2M
Regulation and maintenance of thiyl radicals in enzyme catalysis and protein degradationR35GM154993 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Brandon Greene · 2024 to 2026
$1.1M
NIGMS NIH HHS R35 GM147557NIGMS NIH HHS R35 GM152049NIGMS NIH HHS R35 GM154993
6 · The paper itself

Abstract

Cytochrome P450s (CYPs) are a superfamily of thiolate-ligated heme metalloenzymes principally responsible for the hydroxylation of unactivated C-H bonds. The lower-axial cysteine is an obligatory and universally conserved residue for the CYP enzyme class. Herein, we challenge this paradigm by systematically identifying non-canonical CYPs (ncCYPs) that do not harbor a cysteine ligand. Our bioinformatic search reveals 20 distinct ncCYP families with diverse ligands encoded in microbial genomes. We characterize a native serine-ligated CYP with a high-spin ferric resting state. Its crystal structure clearly shows a typical CYP fold and a serine alkoxide as a lower axial heme ligand. In addition, we report the discovery and characterization of the first native selenocysteine-ligated CYP in nature. Our findings radically expand the CYP metalloenzyme family.

Identifiers

PMID39763895
PMCPMC11703216

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.