Evidence map›Paper›PMID 39763868›Full record

ArticlebioRxiv : the preprint server for biology2024

Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation.

Haiping Ouyang, Cindy Y How, Xiaorong Wang, Clinton Yu, Ang Luo, Lan Huang, Yue Chen

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Haiping OuyangDepartment of Biochemistry, Molecular Biology and Biophysics, the University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Cindy Y HowDepartment of Biochemistry, Molecular Biology and Biophysics, the University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Xiaorong WangDepartment of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA.
Clinton YuDepartment of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA.
Ang LuoDepartment of Biochemistry, Molecular Biology and Biophysics, the University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Lan HuangDepartment of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA.
Yue ChenDepartment of Biochemistry, Molecular Biology and Biophysics, the University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.

Funding

Supplement: Advancing Proteomics Technologies to Decipher the Ubiquitin-Proteasome SystemR35GM145249 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Lan Huang · 2022 to 2026
$3.2M
Functional Dissection of Metabolic-Sensing Proline Hydroxylation PathwaysR35GM124896 · NIGMS · UNIVERSITY OF MINNESOTA · PI Yue Chen · 2017 to 2026
$3.1M
NIGMS NIH HHS R35 GM124896NIGMS NIH HHS R35 GM145249
6 · The paper itself

Abstract

Prolyl Hydroxylase Domain protein 2 (PHD2) targets Hypoxia Inducible Factor alpha subunits (HIFα) for oxygen-dependent proline hydroxylation that leads to subsequent ubiquitination and degradation of HIFα. In addition to HIF proteins, growing evidence suggested that PHD2 may exert its multifaceted function through hydroxylase-dependent or independent activities. Given the critical role of PHD2 in diverse biological processes, it is important to comprehensively identify potential PHD2 interacting proteins. In this study, we engineered HeLa cells that stably express HTBH-tagged PHD2 to facilitate the identification of PHD2 interactome. Using DSSO-based cross-linking mass spectrometry (XL-MS) technology and LC-MS

Indexed as

COPS9crosslinkingCSN complexDSSOEGLN1interactome analysislabel-free quantificationPHD2

Identifiers

PMID39763868
PMCPMC11702602

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.