Evidence map›Paper›PMID 39763729›Full record

ArticlebioRxiv : the preprint server for biology2024

A bacterial NLR-related protein recognizes multiple unrelated phage triggers to sense infection.

Emily M Kibby, Laurel K Robbins, Amar Deep, Nathan K Min, Lindsay A Whalen, Toni A Nagy, Layla Freeborn, Kevin D Corbett, Aaron T Whiteley

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Emily M KibbyDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.ORCID 0000-0002-5506-4576
Laurel K RobbinsDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.ORCID 0000-0001-6662-2783
Amar DeepDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA.ORCID 0000-0003-2003-2889
Nathan K MinDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA.
Lindsay A WhalenDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.
Toni A NagyDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.ORCID 0000-0002-2739-3540
Layla FreebornResearch Computing, Office of Information Technology, University of Colorado Boulder, Boulder, CO, USA.ORCID 0000-0002-3299-4666
Kevin D CorbettDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA.ORCID 0000-0001-5854-2388
Aaron T WhiteleyDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.ORCID 0000-0002-0075-7519

Funding

TRAINING IN SIGNAL TRANSDUCTION &CELL CYCLE REGULATIONT32GM008759 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI AHN, NATALIE G. · 2000 to 2020
$5.9M
Predoctoral Training Program in Signaling and Cellular Regulation INCLUDE Down Syndrome SupplementT32GM142607 · NIGMS · UNIVERSITY OF COLORADO · PI Sabrina Leigh Spencer, Tin Tin Su · 2021 to 2026
$3.6M
Molecular mechanisms of nucleic acid recognition and maintenance in meiosis and innate immunityR35GM144121 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett · 2022 to 2026
$2.7M
Interdisciplinary Predoctoral Training in Molecular BiophysicsT32GM145437 · NIGMS · UNIVERSITY OF COLORADO · PI JOSEPH J FALKE · 2022 to 2026
$2.5M
Research Supplements to Promote Diversity in Health-Related Research (Admin Supp - Clinical Trial Not Allowed)DP2AT012346 · NCCIH · UNIVERSITY OF COLORADO · PI WHITELEY, AARON THOMAS · 2022 to 2025
$2.4M
NCCIH NIH HHS DP2 AT012346NIGMS NIH HHS R35 GM144121NIGMS NIH HHS T32 GM008759NIGMS NIH HHS T32 GM142607NIGMS NIH HHS T32 GM145437
6 · The paper itself

Abstract

Immune systems must rapidly sense viral infections to initiate antiviral signaling and protect the host. Bacteria encode >100 distinct viral (phage) defense systems and each has evolved to sense crucial components or activities associated with the viral lifecycle. Here we used a high-throughput AlphaFold-multimer screen to discover that a bacterial NLR-related protein directly senses multiple phage proteins, thereby limiting immune evasion. Phages encoded as many as 5 unrelated activators that were predicted to bind the same interface of a C-terminal sensor domain. Genetic and biochemical assays confirmed activators bound to the bacterial NLR-related protein at high affinity, induced oligomerization, and initiated signaling. This work highlights how

Indexed as

AlphaFold-multimerAVAST systemNACHTphage defensesensingSTAND

Identifiers

PMID39763729
PMCPMC11702601

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.