Evidence map›Paper›PMID 39762885›Full record

ArticleDiagnostic pathology2025

Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst.

Osvaldo Rodrigues de Souza Neto, Antonia Taiane Lopes de Moraes, Hellen Thais Fuzii, Antonio Guilherme Maneschy Faria, Vanessa Morais Freitas, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves, João de Jesus Viana Pinheiro

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Article in Diagnostic pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Osvaldo Rodrigues de Souza NetoCell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.
Antonia Taiane Lopes de MoraesOral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, 13414-903, Brazil. taiane_moraes12@hotmail.com.
Hellen Thais FuziiImmunopathology Laboratory, Tropical Medicine Institute, Federal University of Pará, Av Generalíssimo Deodoro, 92 Umarizal, Belém, PA, Brazil.
Antonio Guilherme Maneschy FariaSchool of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.
Vanessa Morais FreitasDepartment of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Maria Sueli da Silva KataokaCell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.
Sérgio de Melo AlvesCell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.
João de Jesus Viana PinheiroCell Culture Laboratory, School of Dentistry, Federal University of Para, Rua Augusto Correa, 01 Guama, Belem, PA, 66075110, Brazil.

Funding

Higher Education Personnel Improvement Coordination (CAPES), Ministry of Education, Brazil Finance code 001 - Grant number 88887.640723/2021-00
6 · The paper itself

Abstract

backgroundConsidering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst.

methodsImmunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs).

resultsThe immunohistochemical expression of these proteins was predominantly cytoplasmic and granular across all samples. In epithelial tissue, the immunoexpression of aggrecan and versican was higher in OKC (p < 0.05) compared to DF. Comparing the expression of proteins between the OKC epithelium and the cystic capsule, it was observed that all molecules were more expressed in the epithelium (p < 0.001). RT-PCR confirmed the expression of ADAMTS-1 and proteoglycans in all samples.

conclusionADAMTS-1, aggrecan, brevican, and versican were expressed in all samples with a granular and cytoplasmic pattern. RT-PCR confirmed their presence in both OKC and DF, but only aggrecan and versican exhibited significantly higher levels in OKC (p < 0.05). Protein expression was notably greater in the epithelial component of OKC. These findings underscore the potential role of these proteins in the biological behavior of OKC.

Indexed as

ADAMTS1 ProteinAggrecansImmunohistochemistryOdontogenic CystsVersicansAdolescentAdultBiglycanDental SacFemaleHumansMaleYoung AdultADAMTS1 ProteinADAMTS1 protein, humanAggrecansBGN protein, humanBiglycanVCAN protein, humanVersicansADAMTS-1ImmunohistochemistryOdontogenic keratocystProteoglycans

Identifiers

PMID39762885
PMCPMC11705738

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.