Evidence map›Paper›PMID 39762520›Full record

ArticleActa parasitologica2025

Occurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye.

Omer Faruk Sahin, Ufuk Erol, Osman Furkan Urhan, Husnu Furkan Sakar, Kursat Altay

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Article in Acta parasitologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Omer Faruk SahinDepartment of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye. ofsahin@cumhuriyet.edu.tr.ORCID http://orcid.org/0000-0002-3230-504X
Ufuk ErolDepartment of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye.ORCID http://orcid.org/0000-0002-6766-1335
Osman Furkan UrhanRepublic of Türkiye Ministry of Agriculture and Forestry, General Directory Meat and Milk Board, Sivas Meat Processing Plant, 58380, Sivas, Türkiye.ORCID http://orcid.org/0000-0002-2834-3051
Husnu Furkan SakarDepartment of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye.ORCID http://orcid.org/0009-0009-4647-9321
Kursat AltayDepartment of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye.ORCID http://orcid.org/0000-0002-5288-1239

Funding

Scientific Research Project Fund of Sivas Cumhuriyet University V-2024-136
6 · The paper itself

Abstract

purposeCryptosporidium spp. and Giardia duodenalis are zoonotic protozoan parasites that are widely seen in domestic and wild animals worldwide. While these pathogens, which affect the digestive system of the hosts, cause high economic losses in animal breeding, they are also considered an important public health problem. In recent years, molecular-based studies revealed that 120 genotypes belonging to 44 Cryptosporidium species and eight G. duodenalis assemblages (G. duodenalis A-H) circulate among hosts. The aim of the study was to determine the presence and prevalence of cryptosporidiosis and giardiosis in buffaloes, for which there was only one previous study on the subject in Türkiye.

methodsIn this study, Cryptosporidium spp. and Giardia duodenalis were researched in water buffaloes using polymerase chain reaction (PCR) and DNA sequencing. A total of 510 water buffalo stool samples were obtained from Sivas province, an important water buffalo breeding center in Türkiye.

resultsCryptosporidium spp. were detected in 20 samples (3.92%), whereas five samples (0.98%) were found to be infected with G. duodenalis. DNA sequence analyses of 18S rRNA and β-giardin genes revealed that five Cryptosporidium species, C. occultus (n = 1), C. andersoni (n = 1), C. ryanae (n = 16), C. parvum (n = 1), and C. bovis (n = 1), and G. duodenalis assemblages E were circulated in water buffaloes in Türkiye, respectively. In this work, C. ryanae was the most prevalent Cryptosporidium species, and DNA sequence analyses of these samples showed that 100% nucleotide identities were present between them. Cryptosporidium occultus (PP754270), C. andersoni (PP754271), C. ryanae (PP754272-PP754279, PP754281-PP754285, PP754287-PP754289), C. parvum (PP754280), and C. bovis (PP754286) obtained from water buffaloes in this study shared 98.59-100%, 99.88-100%, 99.49-100%, 99.62-100%, and 99.87-100% nucleotide similarity with isolates present in GeneBank, respectively. In addition, G. duodenalis (PP798352-PP798356) isolates had 99.56-100% (β-giardin) nucleotide identities with G. duodenalis isolates.

conclusionThe existence of cryptosporidiosis (the five species) in water buffaloes was reported for the first time in the country. Moreover, one species (C. occultus) has been reported for the first time in Türkiye.

Indexed as

BuffaloesCryptosporidiosisCryptosporidiumFecesGiardia lambliaGiardiasisRNA, Ribosomal, 18SAnimalsDNA, ProtozoanGenotypePhylogenyPolymerase Chain ReactionPrevalenceProtozoan ProteinsSequence Analysis, DNADNA, ProtozoanProtozoan ProteinsRNA, Ribosomal, 18SCryptosporidiumDNA sequencingGiardia duodenalisPCRTürkiyeWater Buffalo

Identifiers

PMID39762520

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.