Evidence map›Paper›PMID 39762448›Full record

ArticleScientific reports2025

Knockout or inhibition of DHPS suppresses ovarian tumor growth and metastasis by attenuating the TGFβ pathway.

Guannan Zhao, Xinxin Zhao, Ziping Liu, Baojin Wang, Peixin Dong, Hidemichi Watari, Lawrence M Pfeffer, Gabor Tigyi, Wenjing Zhang, Junming Yue

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Guannan ZhaoDepartment of Pathology and Laboratory Medicine, Collage of Medicine, the University of Tennessee Health Science Center, Memphis, TN, 38163, United States.
Xinxin ZhaoDepartment of Obstetrics and Gynecology, The Third Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.
Ziping LiuDepartment of Obstetrics and Gynecology, The Third Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.
Baojin WangDepartment of Obstetrics and Gynecology, The Third Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.
Peixin DongDepartment of Obstetrics and Gynecology, Hokkaido University School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
Hidemichi WatariDepartment of Obstetrics and Gynecology, Hokkaido University School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
Lawrence M PfefferDepartment of Pathology and Laboratory Medicine, Collage of Medicine, the University of Tennessee Health Science Center, Memphis, TN, 38163, United States.
Gabor TigyiDepartment of Physiology, Collage of Medicine, the University of Tennessee Health Science Center, Memphis, TN, 38163, USA.
Wenjing ZhangDepartment of Genetics, Genomics & Informatics, Collage of Medicine, University of Tennessee Health Science Center, Memphis, TN, 38163, USA. wzhang67@uthsc.edu.
Junming YueDepartment of Pathology and Laboratory Medicine, Collage of Medicine, the University of Tennessee Health Science Center, Memphis, TN, 38163, United States. jyue@uthsc.edu.

Funding

Therapeutic targeting of the SWI/SNF chromatin remodeler to regulate GBM chemosensitivityR01CA281977 · NCI · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI DUANE D MILLER, LAWRENCE MARC PFEFFER · 2023 to 2026
$2.0M
Role of MTF1 in ovarian cancerR21CA216585 · NCI · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI YUE, JUNMING · 2018 to 2019
$385k
National Cancer Institute, United States R21CA216585-01A1NCI NIH HHS R01 CA281977NCI NIH HHS R21 CA216585
6 · The paper itself

Abstract

Deoxyhypusine synthase (DHPS) is an enzyme encoded by the DHPS gene, with high expression in various cancers, including ovarian cancer (OC). DHPS regulates the translation initiation factor EIF5A, and EIF5A2 knockout inhibits OC tumor growth and metastasis by blocking the epithelial-to-mesenchymal transition (EMT) and the TGFβ pathway. In this study, we show that DHPS is amplified in OC patients, and its elevated expression correlates with poor survival. Using lentiviral CRISPR/Cas9 vectors for DHPS knockout, we observed EMT inhibition in SKOV3 and OVCAR8 cells through suppressed hypusination and reduced EIF5A2 expression. Inhibition of DHPS activity with GC7 similarly blocked hypusination and EMT. Disrupting DHPS expression, either genetically or pharmacologically, inhibited primary tumor growth and metastasis in OC mouse models. These findings suggest that targeting DHPS and inhibiting hypusination could be promising strategies for OC treatment.

Indexed as

Epithelial-Mesenchymal TransitionOvarian NeoplasmsOxidoreductases Acting on CH-NH Group DonorsRNA-Binding ProteinsSignal TransductionTransforming Growth Factor betaAnimalsCell Line, TumorCell ProliferationEukaryotic Translation Initiation Factor 5AFemaleGene Expression Regulation, NeoplasticGene Knockout TechniquesHumansMiceNeoplasm Metastasisdeoxyhypusine synthaseEukaryotic Translation Initiation Factor 5AOxidoreductases Acting on CH-NH Group DonorsPeptide Initiation FactorsRNA-Binding ProteinsTransforming Growth Factor betaDHPSEIF5AEMTGC7HypusinationMetastasisOrthotopic OC mouse modelsOvarian cancerTGFβ

Identifiers

PMID39762448
PMCPMC11704301

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.