Evidence map›Paper›PMID 39760916›Full record

ArticleMicrobial ecology2025

Predators in the Dark: Metabarcoding Reveals Arcellinida Communities Associated with Bat Guano, Endemic to Dinaric Karst in Croatia.

Ángel García-Bodelón, Najla Baković, Emilio Cano, Fernando Useros, Enrique Lara, Rubén González-Miguéns

Abstract read
In one paragraph

Article in Microbial ecology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ángel García-BodelónReal Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain. agbodelon@rjb.csic.es.
Najla BakovićLaboratory for Flora, Fauna and Habitats, DVOKUT-ECRO Ltd., Zagreb, Croatia.
Emilio CanoReal Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain.
Fernando UserosReal Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain.
Enrique LaraReal Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain.
Rubén González-MiguénsReal Jardín Botánico (RJB-CSIC), C/ Moyano 1, 28014, Madrid, Spain. ruben.miguens@ibe.upf-csic.es.

Funding

Comunidad de Madrid (Spain) Garantía Juvenil CM 2021Croatian Ministry of Economy and Sustainable Development Class: UP/I-612-07/21-48/170, Ref. No.: 517-10-1-1-21-3, 16.07.2021Spanish Ministry of Science, Innovation and Universities PID2021-128499NB-I00
6 · The paper itself

Abstract

Karst caves, formed from the dissolution of soluble rocks, are characterized by the absence of photosynthetic activity and low levels of organic matter. Organisms evolve under these particular conditions, which causes high levels of endemic biodiversity in both macroorganism and microbes. Recent research has highlighted the presence of testate amoebae (Arcellinida) group in cave environments. This study investigates the diversity of Arcellinida in Dinaric karstic caves in Croatia, a global diversity hotspot, focusing on the influence of bat guano on community structure. Sediment samples were collected from two independent hydrosystems, and a metabarcoding approach was used to assess Arcellinida diversity at specific and intraspecific levels, using Arcellinid-specific primers to amplify the mitochondrial cytochrome oxidase subunit I (COI) region. Results reveal a significant impact of guano on both specific and intraspecific diversity of Arcellinida. Communities in guano-rich sites displayed higher diversity, abundance, and the presence of unique OTUs and genetic variants not observed in other habitats, highlighting the crucial role of bats as ecosystem engineers. In contrast, sites without guano hosted communities with low abundance and reduced biodiversity. These differences suggest the existence of guano-associated Arcellinida communities. This study provides new insights into the biodiversity of subterranean ecosystems and the ecological roles of Arcellinida in karstic environments.

Indexed as

BiodiversityCavesChiropteraDNA Barcoding, TaxonomicAnimalsChironomidaeCroatiaEcosystemElectron Transport Complex IVFecesGeologic SedimentsPhylogenyElectron Transport Complex IVArcellinidaBat guanoBiodiversityKarst cavesMetabarcoding

Identifiers

PMID39760916
PMCPMC11703892

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.