Evidence map›Paper›PMID 39760794›Full record

ArticleMolecular biology reports2025

Aqueous and ethanolic extracts of Moringa oleifera leaves induce selective cytotoxicity in Raji and Jurkat cell lines by activating the P21 pathway independent of P53.

Leila Rajabi, Mostafa Ebrahimdoost, Seyed Amin Mohammadi, Hamed Soleimani Samarkhazan, Gholamreza Khamisipour, Mojtaba Aghaei

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed.

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  19. Exploring the Functional Properties of Leaves ofAntioxidants (Basel, Switzerland) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Leila RajabiDepartment of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, 7518759577, Iran.
Mostafa EbrahimdoostDepartment of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, 7518759577, Iran.ORCID https://orcid.org/0000-0003-2558-4965
Seyed Amin MohammadiDepartment of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, 7518759577, Iran.ORCID https://orcid.org/0000-0002-3500-7822
Hamed Soleimani SamarkhazanDepartment of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.ORCID http://orcid.org/0000-0003-1045-7613
Gholamreza KhamisipourDepartment of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, 7518759577, Iran. khamisipourgholamreza@gmail.com.ORCID https://orcid.org/0000-0002-5773-9012
Mojtaba AghaeiStudent Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. mojtabaaghaei745@gmail.com.ORCID https://orcid.org/0000-0002-6382-6657

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe traditional use of Moringa oleifera (MO), an essential food source in Africa and Asia, to cure various diseases dates back thousands of years. This study examines the aqueous and ethanolic leaf extracts of MO's in vitro anti-leukemia capabilities.

methodsAfter preparing aqueous and ethanolic MO leaf extracts, cells were treated with various concentrations for 48 h. Cell viability was measured via MTT assay, and apoptotic pathways were analyzed using flow cytometry and Annexin V-PI staining. Following RNA extraction and cDNA synthesis, cells were exposed to the IC50 (150 µg/ml) for 48 h. Real-time PCR assessed the expression of P21, P53, BCL2, and Survivin genes. Peripheral blood mononuclear cells (PBMCs) served as the control group.

resultsMO aqueous and ethanol extracts showed cytotoxicity, with cancer cells being more sensitive. Flow cytometry confirmed higher apoptotic activity in Jurkat and Raji cells compared to PBMCs. The extracts increased P21 expression in Jurkat cells but did not significantly affect P53, BCL2, or Survivin. Similarly, in Raji cells, P21, BCL2, and Survivin were elevated, while P53 remained unchanged. Gene expression in healthy PBMCs was unaffected by the extracts.

conclusionThis study shows that leukemia cells (Raji and Jurkat) are more sensitive to MO's aqueous and ethanolic extracts than healthy cells. The results suggest developing MO extracts as a cutting-edge leukemia treatment.

Indexed as

ApoptosisCell SurvivalCyclin-Dependent Kinase Inhibitor p21Moringa oleiferaPlant ExtractsPlant LeavesTumor Suppressor Protein p53Cell Line, TumorEthanolHumansJurkat CellsSignal TransductionCDKN1A protein, humanCyclin-Dependent Kinase Inhibitor p21EthanolPlant ExtractsTP53 protein, humanTumor Suppressor Protein p53ApoptosisJurkat cellsLeukemiaMoringa oleiferaRaji cells

Identifiers

PMID39760794

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