Evidence map›Paper›PMID 39759271›Full record

ArticleHeliyon2024

Antimicrobial-resistant genes in feces from otters (

Aurora Guizado-Batista, Andrea Porres-Camacho, Seiling Vargas-Villalobos, Manuel Cortez-Martínez, Rodolfo Umaña-Castro, Carolina Sancho-Blanco, Frank Solano-Campos, Francisco Quesada-Alvarado, Manuel Spínola-Parallada, Alexander Madrigal-Mora and 5 more

Abstract read
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Antimicrobial resistance genes in wild coyotes (One health (Amsterdam, Netherlands) · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Aurora Guizado-BatistaUniversidad Nacional, Costa Rica. Posgrado Regional en Ciencias Veterinarias Tropicales (PCVET), Costa Rica.
Andrea Porres-CamachoUniversidad Nacional, Costa Rica. Posgrado Regional en Ciencias Veterinarias Tropicales (PCVET), Costa Rica.
Seiling Vargas-VillalobosUniversidad Nacional, Costa Rica. Instituto Regional de Estudios en Sustancias Tóxicas (IRET), Costa Rica.
Manuel Cortez-MartínezUniversidad Nacional, Costa Rica. Posgrado Regional en Ciencias Veterinarias Tropicales (PCVET), Costa Rica.
Rodolfo Umaña-CastroUniversidad Nacional, Costa Rica. Laboratorio de Análisis Genómico (LAGen), Escuela de Ciencias Biológicas, Costa Rica.
Carolina Sancho-BlancoUniversidad Nacional, Costa Rica. Laboratorio de Análisis Genómico (LAGen), Escuela de Ciencias Biológicas, Costa Rica.
Frank Solano-CamposUniversidad Nacional, Costa Rica. Laboratorio de Análisis Genómico (LAGen), Escuela de Ciencias Biológicas, Costa Rica.
Francisco Quesada-AlvaradoUniversidad Nacional, Costa Rica. Instituto Regional de Estudios en Sustancias Tóxicas (IRET), Costa Rica.
Manuel Spínola-ParalladaUniversidad Nacional, Costa Rica. Instituto Internacional de Conservación y Manejo de Vida Silvestre (ICOMVIS), Costa Rica.
Alexander Madrigal-MoraInstituto Costarricense de Electricidad (ICE), Costa Rica.
Adonay Jiménez-SerranoUniversidad Nacional, Costa Rica. Laboratorio de Análisis Genómico (LAGen), Escuela de Ciencias Biológicas, Costa Rica.
Joshua Vargas-CalvoUniversidad Nacional, Costa Rica. Laboratorio de Análisis Genómico (LAGen), Escuela de Ciencias Biológicas, Costa Rica.
Jenny Villalobos-SequeiraUniversidad Nacional, Costa Rica. Instituto de Estudios Sociales en Población (IDESPO), Costa Rica.
Kari Brossard StoosDepartment of Health Sciences & Public Health, Ithaca College, Ithaca, NY 14850, United States.
Kinndle Blanco-PeñaUniversidad Nacional, Costa Rica. Instituto Regional de Estudios en Sustancias Tóxicas (IRET), Costa Rica.

Funding

International Training in Environmental Health over the LifespanD43TW011403 · FIC · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI CLAUDIO, LUZ · 2020 to 2024
$1.3M
FIC NIH HHS D43 TW011403
6 · The paper itself

Abstract

Antimicrobial resistance poses a growing threat to human health, yet its implications for wildlife remain a subject of ongoing research. River otters inhabiting the Peñas Blancas River face exposure to various anthropogenic activities in their habitat, potentially leading to the accumulation of antibiotic-resistant genes (ARGs) with unknown consequences for their health. This study aimed to identify specific ARGs in otter feces from this river basin, employing quantitative polymerase chain reaction (qPCR), DNA sequencing of ARGs, and phylogenetic analysis techniques. Over the period from 2019 to 2022, we collected 102 fecal samples from otters through the Peñas Blancas River watershed, spanning its upper and middle basins. We assessed the bacterial presence via the 16S rRNA gene through qPCR analysis and screened for 12 ARGs. Sequences of 16 ARG-positive samples were subsequently analyzed using Maximum-likelihood-base taxonomic placement. In total, 56 samples tested positive for the 16S rRNA gene, with 24 exhibiting at least one ARG. Notably, three samples showcased a "multi-resistance microbiome". qPCR analyses identified seven distinct ARGs:

Indexed as

Anthropogenic activitiesAntimicrobial resistanceAquatic ecosystemsMobile genetic elementsMulti-resistance microbiomePhylogenySentinelsWildlife

Identifiers

PMID39759271
PMCPMC11697563

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.